Hibiscus moscheutos L., also known as hardy hibiscus, is valued for its medicinal and ornamental attributes. It is usually propagated via seeds or cuttings. The purpose of this investigation was to develop a dependable micropropagation for H. moscheutos ‘Luna White’. To that end, the effect of four explant types (leaf, root, node, shoot tip) and two growth regulators 6-benzylaminopurine (BA) and meta-Topolin (mT) (6-(3-hydroxybenzylamino) purine) on in vitro growth of H. moscheutos was investigated. Genetic stability of the in vitro grown plants was assessed using flow cytometry, and chromosome count was investigated. No shoots were obtained from leaf or root explants. An efficient protocol for micropropagation of H. moscheutos using two explant types, 2-node and shoot tip explants, and two cytokinins (BA and mT) capable of producing true-to-type regenerants was established. Both BA and mT can be used at 2 μM or 4 μM using either 2-node or shoot tip explants. No significant difference was found between the nuclear DNA contents of seed-derived and in vitro grown plants (P < 0.05). The mean 2C DNA and monoploid 1Cx-values of seed-derived plants were 3.25 ± 0.08 pg and 1.62 ± 0.04 pg, respectively, compared with 3.26 ± 0.06 pg and 1.63 ± 0.02 pg, respectively, for in vitro grown plants. The chromosome number of both seed-derived plants and regenerants was determined to be 2n = 2x = 38. The mature regenerants obtained were fertile and phenotypically similar to seed-derived plants.

Hibiscus moscheutos L.，也被称为耐寒木槿，因其药用和观赏特性而受到重视。它通常通过种子或插条繁殖。这项调查的目的是为H. moscheutos 'Luna White'开发一种可靠的微繁殖。为此，四种外植体类型（叶、根、节、茎尖）和两种生长调节剂 6-苄氨基嘌呤 (BA) 和间位托蛋白 ( mT )（6-（3-羟基苄氨基）嘌呤）对体外的影响研究了H. moscheutos 的生长。体外遗传稳定性使用流式细胞术评估生长的植物，并研究染色体计数。没有从叶或根外植体获得芽。建立了使用两种外植体类型、2 节点和茎尖外植体以及两种能够产生真正类型再生体的细胞分裂素（BA 和m T）微繁殖H. moscheutos的有效方案。BA 和mT都可以使用 2 μM 或 4 μM 使用 2 节点或茎尖外植体。种子来源和体外生长的植物的核 DNA 含量之间没有发现显着差异（P< 0.05)。种子来源植物的平均 2C DNA 和单倍体 1Cx 值分别为 3.25 ± 0.08 pg 和 1.62 ± 0.04 pg，而体外生长的植物分别为 3.26 ± 0.06 pg 和 1.63 ± 0.02 pg 。种子衍生植物和再生体的染色体数目确定为 2 n = 2 x = 38。获得的成熟再生体可育且表型与种子衍生植物相似。