The Journal of Membrane Biology ( IF 2.3 ) Pub Date : 2021-09-04 , DOI: 10.1007/s00232-021-00200-2 Akshay Kumar 1 , Pawan Kumar Maurya 1
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Abstract
Increasing interest has recently focused on determining whether quercetin may exert anti-aging properties or not? The objective of this study was determination of Na+, K+ -ATPase activity in quercetin-treated red blood cells during human aging. The study was carried out on human blood samples. The subjects were divided into different age groups, young, middle, and old. The effects of quercetin were evaluated by determining Na+, K+ -ATPase activity by co-incubating the red blood cells in presence of quercetin (10−6 M to 10−3 M final concentration). Quercetin causes 15% increase in Na+, K+ -ATPase activity at 10−4 M and 17% at 10−3 M as compared to the young control age group. The effect was insignificant at 10−5 M (7%) and 10−6 M (5%) in the young age group. Quercetin showed significant increase at 10−6 M to 10−3 M in Na+, K+ -ATPase activity as compared to the middle control age group. A significant increase in Na+, K+ -ATPase activity was observed at all concentrations [10−6 M (31%), 10−5 M (39%), 10−4 M (51%), and 10−3 M (61%)] in elderly population. We believe that these findings will help in further research against oxidative stress in red blood cells.
Graphic Abstract
中文翻译：
槲皮素在人类衰老过程中减轻红细胞膜结合的 Na+、K+-ATP 酶转运蛋白
摘要
最近越来越多的兴趣集中在确定槲皮素是否可以发挥抗衰老作用？本研究的目的是测定 人类衰老过程中槲皮素处理的红细胞中的 Na +、K + -ATP 酶活性。该研究是在人体血液样本上进行的。受试者被分为不同年龄组，青年、中年和老年。通过在槲皮素（10 -6 M 至 10 -3 M 终浓度） 存在下共孵育红细胞来测定 Na +、K + -ATP酶活性来评估槲皮素的效果。槲皮素在 10 -4时导致 Na +、K + -ATP 酶活性增加 15% 与年轻对照组相比， M 和 10 -3 M 时为 17% 。 在年轻组中，在 10 -5 M (7%) 和 10 -6 M (5%) 时效果不显着。 与中间对照组相比，槲皮素在 10 -6 M 至 10 -3 M 时 Na +、K + -ATP酶活性显着增加。在 所有浓度[10 -6 M (31%)、10 -5 M (39%)、10 -4 M (51%) 和10 -3 M (61%)] 在老年人群中。我们相信这些发现将有助于进一步研究红细胞中的氧化应激。
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