Recently, a new technology of low-energy ultrashort-pulsed electron beam (UPEB) accelerators has been developed opening new directions for radiobiology and biomedical research. The purpose of this study was to reveal the lethal dose, LD50 (lethal dose, 50%) delivered by low-energy UPEB whole-body exposure on an organismal level. Wistar rats were exposed to low-energy UPEB whole-body irradiation with different doses and pulse repetition rates to find the LD50 and in silico computer simulations were performed to conduct numerical dose calculations. Survival rate, body weight and water consumption were monitored over the 30-day observation period postirradiation. The LD50 was observed after a 2 Gy dose and pulse repetition rate of 2 Hz. In this group, 50% of the animals survived 30 days postirradiation. The groups of animals exposed to low-energy UPEB radiation at higher doses and pulse repetition rates demonstrated higher mortality rates. We demonstrated that the LD50 dose for the low-energy UPEB whole body irradiation in Wistar rats corresponds to 2 Gy with a pulse repetition rate of 2 Hz. Moreover, we showed that the pulse repetition rate is a very important parameter in the experiments with UPEB and should be assessed in the experiments with such kind of novel irradiation sources.

最近，一项低能超短脉冲电子束 (UPEB) 加速器新技术的开发为放射生物学和生物医学研究开辟了新的方向。本研究的目的是揭示低能量 UPEB 全身暴露在生物体水平上的致死剂量 LD50（致死剂量，50%）。将 Wistar 大鼠暴露于不同剂量和脉冲重复率的低能量 UPEB 全身照射，以找到 LD50 和计算机模拟进行了计算机模拟以进行数值剂量计算。在照射后 30 天的观察期内监测存活率、体重和饮水量。在 2 Gy 剂量和 2 Hz 脉冲重复率后观察到 LD50。在该组中，50% 的动物在辐射后 30 天存活。暴露于高剂量和高脉冲重复率的低能量 UPEB 辐射的动物组表现出更高的死亡率。我们证明了 Wistar 大鼠低能量 UPEB 全身照射的 LD50 剂量对应于 2 Gy，脉冲重复率为 2 Hz。此外，我们表明脉冲重复率在 UPEB 实验中是一个非常重要的参数，应该在这种新型辐射源的实验中进行评估。