Ultrasensitive and Reusable Graphene Oxide-Modified Double-Interdigitated Capacitive (DIDC) Sensing Chip for Detecting SARS-CoV-2,ACS Sensors

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Ultrasensitive and Reusable Graphene Oxide-Modified Double-Interdigitated Capacitive (DIDC) Sensing Chip for Detecting SARS-CoV-2
ACS Sensors ( IF 8.2 ) Pub Date : 2021-09-03 , DOI: 10.1021/acssensors.1c01437
Parshant Kumar Sharma _{1,

2} , Eun-Seong Kim _{1,

2} , Sachin Mishra _{1,

2,

3} , Enkhzaya Ganbold _{1,

2} , Ryun-Sang Seong _{1,

2} , Ajeet Kumar Kaushik ₄ , Nam-Young Kim _{1,

2,

3}

Affiliation

This research reveals the promising functionalization of graphene oxide (GrO)-glazed double-interdigitated capacitive (DIDC) biosensing platform to detect severe acute respiratory syndrome coronavirus (SARS-CoV-2) spike (S1) proteins with enhanced selectivity and rapid response. The DIDC bioactive surface consisting of Pt/Ti featured SiO₂ substrate was fabricated using GrO/EDC-NHS/anti-SARS-CoV-2 antibodies (Abs) which is having layer-by-layer interface self-assembly chemistry method. This electroactive immune-sensing platform exhibits reproducibility and sensitivity with reference to the S1 protein of SARS-CoV-2. The outcomes of analytical studies confirm that GrO provided a desired engineered surface for Abs immobilization and amplified capacitance to achieve a wide detection range (1.0 mg/mL to 1.0 fg/mL), low limit of detection (1 fg/mL) within 3 s of response time, good linearity (18.56 nF/g), and a high sensitivity of 1.0 fg/mL. Importantly, the unique biochip was selective against blood-borne antigens and standby for 10 days at 5 °C. Our developed DIDC-based SARS-CoV-2 biosensor is suitable for point-of-care (POC) diagnostic applications due to portability and scaling-up ability. In addition, this sensing platform can be modified for the early diagnosis of severe viral infections using real samples.

中文翻译：

用于检测 SARS-CoV-2 的超灵敏且可重复使用的氧化石墨烯改性双叉指电容 (DIDC) 传感芯片

这项研究揭示了氧化石墨烯 (GrO) 釉面双叉指电容 (DIDC) 生物传感平台在检测严重急性呼吸综合征冠状病毒 (SARS-CoV-2) 尖峰 (S1) 蛋白方面具有前景的功能化，具有增强的选择性和快速响应。由 Pt/Ti 组成的 DIDC 生物活性表面特征为 SiO ₂使用具有逐层界面自组装化学方法的 GrO/EDC-NHS/抗 SARS-CoV-2 抗体 (Abs) 制造基板。该电活性免疫传感平台参照 SARS-CoV-2 的 S1 蛋白表现出可重复性和敏感性。分析研究的结果证实，GrO 为 Abs 固定和放大电容提供了所需的工程表面，以在 3 秒内实现宽检测范围（1.0 mg/mL 至 1.0 fg/mL）、低检测限（1 fg/mL）响应时间、良好的线性 (18.56 nF/g) 和 1.0 fg/mL 的高灵敏度。重要的是，独特的生物芯片对血源性抗原具有选择性，并在 5°C 下待机 10 天。我们开发的基于 DIDC 的 SARS-CoV-2 生物传感器具有便携性和放大能力，适用于即时 (POC) 诊断应用。此外，可以修改该传感平台以使用真实样本对严重病毒感染进行早期诊断。

更新日期：2021-09-24

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