Background

Nuclear factor-κB (NF-κB) has been identified as the major link between inflammation and cancer. Natural agents that inhibit this pathway are essential in attenuating inflammation induced by cancer or chemotherapeutic drugs. High intake of Brassicaceae vegetables has been determined to modulate essential pathways related to chronic diseases. In this study, we investigated the anti-proliferative and anti-inflammatory effects of the indole glucosinolates; indole-3-carbinol (I3C) and its metabolite 3,3-diindolylmethane (DIM) on the inflammatory biomarkers and miRNAs controlling the NF-κB pathway.

Methods and results

In our study, we inoculated Ehrlich ascites carcinoma (EAC) cells in female albino mice, which increased their packed cell volume and induced a significant increase in the levels of several cytokines and inflammatory biomarkers (NF-κB IL-6, IL-1b, TNF-α, and NO). A significant elevation in inflammatory-medicated miRNAs (miR-31 and miR-21) was also noted. Treatment with 5-fluorouracil (5-FU) significantly reduced packed cell volume and viable cell count. However, it was accompanied by a significant increase in the levels of inflammatory markers and expression of miR-31 and miR-21. Nevertheless, although treatment with indoles (I3C and DIM) significantly reduced the packed cell volume and viable cell count, their prominent effect was the marked reduction of all inflammatory biomarkers compared to both the EAC untreated group and the EAC group treated with 5-FU. Moreover, the anti-inflammatory effect of I3C or DIM was accompanied by a significant decrease in the expression of miR-31 and miR-21.

Conclusion

Our findings have; therefore, revealed that I3C and DIM have strong anti-inflammatory effects, implying that their use as a co-treatment with chemotherapeutic drugs can effectively improve the anti-tumor effect of chemotherapeutic drugs.

中文翻译：

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吲哚硫代葡萄糖苷通过调节炎症标志物和 miRNA 对艾氏腹水癌细胞具有抗炎作用

背景

核因子-κB (NF-κB) 已被确定为炎症和癌症之间的主要联系。抑制该途径的天然药物对于减轻由癌症或化疗药物引起的炎症至关重要。已确定大量摄入十字花科蔬菜可调节与慢性疾病相关的基本途径。在这项研究中，我们研究了吲哚芥子油苷的抗增殖和抗炎作用。indole-3-carbinol (I3C) 及其代谢物 3,3-diindolylmethane (DIM) 对炎症生物标志物和控制 NF-κB 通路的 miRNA 的影响。

方法和结果

在我们的研究中，我们在雌性白化病小鼠中接种了 Ehrlich 腹水癌 (EAC) 细胞，这增加了它们的堆积细胞体积并诱导了几种细胞因子和炎症生物标志物（NF-κB IL-6、IL-1b、 TNF-α 和 NO)。还注意到炎症药物 miRNA（miR-31 和 miR-21）显着升高。用 5-氟尿嘧啶 (5-FU) 处理显着减少了堆积细胞体积和活细胞计数。然而，它伴随着炎症标志物水平和 miR-31 和 miR-21 表达的显着增加。然而，尽管用吲哚（I3C 和 DIM）处理显着减少了堆积细胞体积和活细胞计数，与 EAC 未治疗组和用 5-FU 治疗的 EAC 组相比，它们的显着效果是所有炎症生物标志物的显着减少。此外，I3C 或 DIM 的抗炎作用伴随着 miR-31 和 miR-21 表达的显着降低。

结论

我们的发现有；因此，揭示了I3C和DIM具有很强的抗炎作用，暗示它们与化疗药物联合使用可以有效提高化疗药物的抗肿瘤作用。