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A Preliminary Survey of the Distribution of Segmented Filamentous Bacteria in the Porcine Gastrointestinal Tract
Current Microbiology ( IF 2.3 ) Pub Date : 2021-09-03 , DOI: 10.1007/s00284-021-02636-0
Łukasz Grześkowiak 1 , Beatriz Martínez-Vallespín 1 , Jürgen Zentek 1 , Wilfried Vahjen 1
Affiliation  

Segmented filamentous bacteria (SFB) are present in various animal species including pigs. The aim of this work was to analyze the occurrence of SFB in different parts of the gastrointestinal tract of piglets of different ages. A total of 377 DNA extracts from stomach, jejunum, ileum, cecum and colon digesta, and from feces collected on different time points, originating from 155 animals, were screened by qPCR method with primers specific for the SFB. SFB sequences were detected in 74 of 377 samples (19.6%) from 155 animals in total. SFB were most abundant in ileum (50.0%), cecum (45.0%), and colon (37.0%), followed by feces (14.6%). SFB prevalence in sows was 12.9% (13/101) and 75.9% (41/54) in individual piglets. Of the 41 SFB-positive piglets, only two samples were from pre-weaning animals, while the rest of samples originated from post-weaning piglets. SFB sequences are abundant in post-weaning piglets, but not in suckling or adult animals. They are most abundant in the ileum and cecum of pigs. Further studies are warranted to reveal the role of SFB in pigs.



中文翻译:

猪胃肠道分段丝状菌分布的初步调查

分段丝状细菌 (SFB) 存在于包括猪在内的各种动物中。本工作旨在分析不同年龄仔猪胃肠道不同部位SFB的发生情况。通过 qPCR 方法使用 SFB 特异性引物筛选来自 155 只动物的胃、空肠、回肠、盲肠和结肠食糜以及来自不同时间点收集的粪便的总共 377 个 DNA 提取物。在来自 155 只动物的 377 个样本中的 74 个(19.6%)中检测到 SFB 序列。SFB 在回肠 (50.0%)、盲肠 (45.0%) 和结肠 (37.0%) 中含量最高,其次是粪便 (14.6%)。母猪的 SFB 患病率为 12.9% (13/101) 和单个仔猪的 75.9% (41/54)。在 41 头 SFB 阳性仔猪中,只有两个样本来自断奶前的动物,而其余的样本来自断奶后的仔猪。SFB 序列在断奶后的仔猪中很丰富,但在哺乳或成年动物中却没有。它们在猪的回肠和盲肠中含量最高。需要进一步的研究来揭示 SFB 在猪中的作用。

更新日期:2021-09-04
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