Inflammation has been known to be involved in the pathogenesis of mastitis. And anti-inflammatory agent is proposed to be a possible efficient therapeutic strategy for mastitis. Corynoline, a bioactive compound extracted from Corydalis bungeana Turcz., has been reported to have anti-inflammatory effect. However, whether corynoline has protective effect against mastitis remains unclear. The aim of this study was to evaluate the protective effect of corynoline on LPS-induced mastitis in mice. Inflammatory cytokine production was measured by ELISA. The proteins of signaling pathways were detected by western blot analysis. The results showed that treatment of corynoline at the doses of 15, 30, and 60 mg/kg significantly attenuated LPS-induced pathological damage of mammary tissues. Corynoline also ameliorated LPS-induced MPO activity, MDA content, and inflammatory cytokine TNF-α and IL-1β production in mammary tissues. LPS-induced NF-κB activation was inhibited by corynoline. Furthermore, our results showed corynoline significantly increased the expression of Nrf2 and the phosphorylation levels of AKT and GSK3β. In conclusion, our results indicated that corynoline protected against LPS-induced mastitis through regulating AKT/GSK3β/Nrf2 signaling pathway, which subsequently led to the inhibition of NF-κB and inflammatory response.

中文翻译：

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Corynoline通过调节AKT/GSK3β/Nrf2信号通路保护脂多糖诱发的乳腺炎

已知炎症与乳腺炎的发病机制有关。并且抗炎剂被认为是一种可能有效的乳腺炎治疗策略。Corynoline，一种从Corydalis bungeana Turcz 中提取的生物活性化合物.，据报道具有抗炎作用。然而，corynoline 是否对乳腺炎有保护作用尚不清楚。本研究的目的是评估 corynoline 对 LPS 诱导的小鼠乳腺炎的保护作用。通过ELISA测量炎症细胞因子的产生。通过蛋白质印迹分析检测信号通路的蛋白质。结果表明，以 15、30 和 60 mg/kg 的剂量处理 corynoline 可显着减轻 LPS 诱导的乳腺组织病理损伤。Corynoline 还改善了 LPS 诱导的 MPO 活性、MDA 含量以及乳腺组织中炎性细胞因子 TNF-α 和 IL-1β 的产生。LPS 诱导的 NF-κB 活化被 corynoline 抑制。此外，我们的结果表明，corynoline 显着增加了 Nrf2 的表达以及 AKT 和 GSK3β 的磷酸化水平。总之，我们的结果表明，corynoline 通过调节 AKT/GSK3β/Nrf2 信号通路来预防 LPS 诱导的乳腺炎，随后导致抑制 NF-κB 和炎症反应。