The paper reports the preparation and characterization of a series of functionalized chitosan nanofibers designed for tissue engineering. Their functionalization was done by imination with two aldehydes with complementary bioactivity, one possessing antimicrobial activity and the other one having potential to enhance it by improving the cell permeability. The degree of imination of the fibers and the dynamic character of the imine units were investigated by FTIR, ¹H NMR and UV–vis spectroscopy, while their morphology was assessed by scanning electron microscopy and polarized light microscopy. The swelling ability and biodegradation were determined in conditions mimicking the physiological environment, i.e. saline buffer and lysozyme medium at 37 °C. Preliminary biocompatibility and antimicrobial properties were evaluated by MTS assay on normal human dermal fibroblasts, and by disk diffusion method against relevant bacterial and fungal reference strains. The fibers showed fast swelling, biodegradation in the presence of lysozyme, biocompatibility and antimicrobial activity. It was concluded that the imination of chitosan nanofibers with two different aldehydes is a promising route towards biomaterials with improved properties for tissue engineering.

该论文报告了一系列用于组织工程的功能化壳聚糖纳米纤维的制备和表征。它们的功能化是通过用两种具有互补生物活性的醛进行亚胺化来完成的，一种具有抗菌活性，另一种具有通过提高细胞渗透性来增强抗菌活性的潜力。通过FTIR研究了纤维的亚胺化程度和亚胺单元的动态特性，¹H NMR 和 UV-vis 光谱，同时通过扫描电子显微镜和偏光显微镜评估它们的形态。溶胀能力和生物降解在模拟生理环境的条件下测定，即37°C 的盐水缓冲液和溶菌酶培养基。通过对正常人真皮成纤维细胞的 MTS 分析和针对相关细菌和真菌参考菌株的圆盘扩散法评估初步的生物相容性和抗菌性能。纤维在溶菌酶存在下表现出快速溶胀、生物降解、生物相容性和抗菌活性。得出的结论是，用两种不同的醛类对壳聚糖纳米纤维进行亚胺化是获得具有改进组织工程特性的生物材料的有希望的途径。