The Theoretical Model, Method, and Applications of Scattering Photon Burst Counting Based on an Objective Scanning Technique,Analytical Chemistry

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The Theoretical Model, Method, and Applications of Scattering Photon Burst Counting Based on an Objective Scanning Technique
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-09-03 , DOI: 10.1021/acs.analchem.1c01834
Chaoqing Dong ₁ , Qing Wang ₁ , Zhenli Xu ₂ , Liyun Deng ₁ , Tian Zhang ₁ , Binglin Lu ₃ , Qin Wang ₃ , Jicun Ren ₁

Affiliation

Scattering photon burst counting (SPBC) is a single-particle detection method, which is based on measuring scattering photon bursting of single nanoparticles through a detection volume of <1 fL. Although SPBC has been used for bioassays and analysis of nanoparticles, it is necessary to establish its theoretical model and develop a new detection mode in order to further enhance its sensitivity and enlarge its application fields. In this paper, we proposed a theoretical model for the confocal SPBC method and developed a novel SPBC detection mode using the fast objective scanning technique. The computer simulations and experiments documented that this model well describes the relation between photon counts and experimental parameters (such as nanoparticle concentration and diameter, temperature, and viscosity). Based on this model, we developed a novel SPBC detection mode by using the fast objective scanning technique. Compared to the current confocal SPBC method, the sensitivity of this new method was significantly increased due to the significantly increased photon counts per sampling time, the linear detection range is from 0.9 to 90 pM, and the limit of detection is reduced to 40 fM for 30 nm gold nanoparticles. Furthermore, this new method was successfully applied to determine the enzyme activity of caspase-3 and evaluate the inhibition effectiveness of some inhibitors.

中文翻译：

基于物镜扫描技术的散射光子暴计数的理论模型、方法及应用

散射光子爆发计数 (SPBC) 是一种单粒子检测方法，它基于通过 <1 fL 的检测体积测量单个纳米粒子的散射光子爆发。虽然SPBC已经用于纳米粒子的生物测定和分析，但需要建立其理论模型并开发新的检测模式，以进一步提高其灵敏度并扩大其应用领域。在本文中，我们提出了共焦 SPBC 方法的理论模型，并使用快速客观扫描技术开发了一种新颖的 SPBC 检测模式。计算机模拟和实验证明，该模型很好地描述了光子计数与实验参数（如纳米粒子浓度和直径、温度和粘度）之间的关系。基于这个模型，我们通过使用快速客观扫描技术开发了一种新颖的 SPBC 检测模式。与目前的共聚焦 SPBC 方法相比，这种新方法的灵敏度显着提高，因为每个采样时间的光子计数显着增加，线性检测范围从 0.9 到 90 pM，检测限降低到 40 fM 30 nm 金纳米粒子。此外，这种新方法已成功应用于测定 caspase-3 的酶活性并评估一些抑制剂的抑制效果。对于 30 nm 金纳米粒子，检测限降低到 40 fM。此外，这种新方法已成功应用于测定 caspase-3 的酶活性并评估一些抑制剂的抑制效果。对于 30 nm 金纳米粒子，检测限降低到 40 fM。此外，这种新方法已成功应用于测定 caspase-3 的酶活性并评估一些抑制剂的抑制效果。

更新日期：2021-09-21

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