It has been found that fibroblast growth factor receptor (FGF-FGFR) signaling can regulate the expression of adipocyte differentiation genes. FGF9 is one of the members of FGFs that mainly binds receptors FGFR2 and FGFR3. FGF9 is highly expressed in the adipose tissue of humans and mice, but there are few reports on the role of FGF9 in goat intramuscular adipocyte differentiation. Therefore, this study explored the effect of FGF9 on adipocyte differentiation through cell culture, interference, and overexpression. The expression of receptors FGFR1–FGFR4 in adipocyte differentiation and their effects on differentiation were detected to screen receptor gene of FGF9. Finally, the interaction between FGF9 and the receptor was tested by cotransfection. Our results showed that FGF9 interacts with FGFR2 to inhibit goat intramuscular adipocyte differentiation by regulating peroxisome proliferator-activated receptor gamma (PPARγ) and preadipocyte factor 1 (Pref1), which is a data support for subsequent pathway research.

中文翻译：

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成纤维细胞生长因子9对山羊肌内脂肪细胞分化的调控

已经发现成纤维细胞生长因子受体（FGF-FGFR）信号可以调节脂肪细胞分化基因的表达。FGF9是FGFs的成员之一，主要结合受体FGFR2和FGFR3。FGF9在人和小鼠的脂肪组织中高表达，但关于FGF9在山羊肌内脂肪细胞分化中作用的报道较少。因此，本研究通过细胞培养、干扰和过表达探索了FGF 9 对脂肪细胞分化的影响。受体FGFR 1- FGFR的表达检测4在脂肪细胞分化中的作用及其对分化的影响，以筛选FGF 9 的受体基因。最后，通过共转染检测FGF 9 与受体的相互作用。我们的研究结果表明，FGF 9 与FGFR 2相互作用，通过调节过氧化物酶体增殖物激活受体γ（PPAR γ）和前脂肪细胞因子1（Pref 1）来抑制山羊肌内脂肪细胞分化，为后续通路研究提供了数据支持。