Mammalian cell line development is a multistep process wherein timelines for developing clonal cells to be used as manufacturing cell lines for biologics production can commonly extend to 9 months when no automation or modern molecular technologies are involved in the workflow. Steps in the cell line development workflow involving single-cell cloning, monoclonality assurance, productivity and stability screening are labor, time and resource intensive when performed manually. Introduction of automation and miniaturization in these steps has reduced the required manual labor, shortened timelines from months to weeks, and decreased the resources needed to develop manufacturing cell lines. This review summarizes the advances, benefits, comparisons and shortcomings of different automation platforms available in the market for rapid isolation of desired clonal cell lines for biologics production.

中文翻译：

---

在用于生物制剂生产的哺乳动物细胞系开发中加速单细胞克隆、单克隆性和早期克隆筛选步骤的高通量和自动化进展

哺乳动物细胞系开发是一个多步骤的过程，其中，当工作流程中不涉及自动化或现代分子技术时，开发用于生物制剂生产细胞系的克隆细胞的开发时间通常可以延长至 9 个月。细胞系开发工作流程中涉及单细胞克隆、单克隆性保证、生产力和稳定性筛选的步骤在手动执行时是劳动、时间和资源密集型的。在这些步骤中引入自动化和小型化减少了所需的体力劳动，将时间从几个月缩短到几周，并减少了开发制造细胞系所需的资源。这篇综述总结了进步、好处、