We have reported an optical indicator displacement assay (IDA) for heparin with a UV-vis absorbance and fluorescence dual-read out based on pyranine/methyl viologen (MV²⁺). Upon introducing heparin, pyranine/MV²⁺ shows a clearly observable increase in UV-vis absorbance and a turn-on of the fluorescence signal. We have demonstrated that the ionic nature of buffers significantly affects the pyranine displacement and the zwitterionic HEPES was most suitable for heparin sensing. After careful screening of experimental conditions, the pyranine/MV²⁺-based optical chemosensor exhibits a fast, sensitive, and selective response toward heparin. It shows dynamic linear concentration of heparin in the ranges of 0.1-40 U·mL^-1 and 0.01-20 U·mL^-1 for the absorptive and fluorescent measurements, respectively, which both cover the clinically relevant levels of heparin. As with the animal experiments, the optical chemosensor has been demonstrated to be selective and effective for heparin level qualification in rat plasma. The chemosensor is readily accessible, cost-effective, and reliable, which holds a great promise for potential application on clinical and biological studies. Furthermore, this IDA system can serve as an IMPLICATION logic gate with a reversible and switchable logical manner.

我们已经报道了一种基于吡喃/甲基紫精 (MV ²⁺ ) 的具有紫外-可见光吸收和荧光双重读出的肝素光学指示剂置换测定 (IDA )。引入肝素后，吡喃/MV ²⁺显示出明显可见的紫外-可见光吸收增加和荧光信号的开启。我们已经证明缓冲液的离子性质显着影响吡喃位移，两性离子 HEPES 最适合肝素传感。在仔细筛选实验条件后，基于吡喃/MV ²⁺的光学化学传感器对肝素表现出快速、灵敏和选择性的响应。它显示了肝素在 0.1-40 U·mL ^-1和 0.01-20 U·mL范围内的动态线性浓度^{-1 分别}用于吸收和荧光测量，它们都涵盖了肝素的临床相关水平。与动物实验一样，光学化学传感器已被证明对大鼠血浆中的肝素水平鉴定具有选择性和有效性。该化学传感器易于获取、经济高效且可靠，在临床和生物学研究中的潜在应用前景广阔。此外，该 IDA 系统可以作为具有可逆和可切换逻辑方式的 IMPLICATION 逻辑门。