The purpose of this study is to investigate the protective effect of γ-oryzanol (ORY) against hepatic ischemia reperfusion (HIR) injury and the potential protective mechanisms of ORY. ORY is an important biologically active ingredient isolated from rice bran oil, which has anti-inflammatory and antiapoptotic effects. However, it is still unknown whether ORY can protect the liver from the HIR damage. In this study, ORY was administered orally for seven days, after which the animals were subjected to liver ischemia for 60 minutes and reperfused for 6 hours. Related indicators were analyzed. The results showed that ORY pretreatment significantly reduced the levels of AST and ALT, relieved hepatocellular damage and apoptosis, and attenuated the exhaustion of SOD and GSH and accumulation of MDA and MPO. Interestingly, ORY treatment could significantly decreased ER stress. Furthermore, ORY pretreatment remarkably reduced the protein expressions of HMGB1, NLRP3, caspase-1 (p20), and IL-1β to protect the liver from I/R-induced inflammasome activation and apoptosis. In conclusion, we demonstrated the potential effect of ORY in modulating oxidative stress, endoplasmic reticulum stress, and inflammasome activation during HIR.

中文翻译：

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膳食补充剂 γ-谷维素通过抑制内质网应激和 HMGB1/NLRP3 炎性体减轻肝缺血再灌注损伤

本研究的目的是研究γ的保护作用-谷维素 (ORY) 对抗肝缺血再灌注 (HIR) 损伤和 ORY 的潜在保护机制。ORY是从米糠油中分离出来的一种重要的生物活性成分，具有抗炎和抗凋亡作用。然而，ORY 能否保护肝脏免受 HIR 损伤仍是未知数。在这项研究中，ORY 口服给药 7 天，之后动物进行肝脏缺血 60 分钟并再灌注 6 小时。分析了相关指标。结果表明，ORY预处理显着降低了AST和ALT水平，减轻了肝细胞损伤和细胞凋亡，减轻了SOD和GSH的消耗以及MDA和MPO的积累。有趣的是，ORY 治疗可以显着降低 ER 应激。此外，β保护肝脏免受 I/R 诱导的炎症小体活化和凋亡。总之，我们证明了 ORY 在 HIR 期间调节氧化应激、内质网应激和炎症小体激活的潜在作用。