School of Chemical Engineering, University of Science and Technology Liaoning, Anshan, Liaoning Province, 114051, P. R. China.
Anshan Tumor Hospital, 339 Shenhua Road, Lishan District, Anshan, Liaoning Province, P. R. China.
Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan, Liaoning Province, 114051, P. R. China.
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, 4072, Australia.
We have developed a 1,8-naphthalimide-based fluorescence probe (NI) for monitoring of the HSO3−/H2O2 regulated cycle. The reversible response was realized by HSO3−-involved 1,4-addition and H2O2-induced elimination reaction with C=C double bond of NI. Monitoring the reversible and dynamic inter-conversion between HSO3− and H2O2in vitro and in vivo has been verified by fluorescence imaging in live HeLa cells, adult zebrafish and nude mice. The successful applications of NI prove that it would be a useful tool for a range of redox-related physiological and pathological investigations in the future.
中文翻译：
一种用于体外和体内可逆检测亚硫酸氢盐和过氧化氢对的新型荧光探针
我们开发了一种基于 1,8-萘二甲酰亚胺的荧光探针 (NI)，用于监测 HSO 3 - /H 2 O 2调节循环。可逆反应是通过 HSO 3 - -涉及 1,4-加成和 H 2 O 2诱导的消除反应与NI 的C=C 双键实现的。已通过活 HeLa 细胞、成年斑马鱼和裸鼠的荧光成像验证了在体外和体内监测 HSO 3 -和 H 2 O 2 之间的可逆和动态相互转换。NI的成功应用 证明它将成为未来一系列氧化还原相关生理和病理研究的有用工具。
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