Here, the regenerative potential of menstrual blood–derived mesenchymal stem cells (MenSCs) was examined on restoration of premature ovarian failure (POF) ovaries in rats' POF model. Freshly isolated CD146⁺ MenSCs using magnetic-activated cell storing method were immediately injected into ovaries of POF rats. Four and eight weeks after cell administration, both ovarian tissues were sampled for histological examination and the expression of fibrosis-related genes. Serum samples were also prepared for hormonal analysis. At the endpoint, mating trials were performed to assess the fertility of POF rats following MenSC transplantation. Histopathological examination revealed the induction of POF after Ceftriaxone injection by increasing atretic follicles and abnormal morphologies. MenSCs transplantation increased the number of normal follicles and coincided with the reduction of follicular atresia. Biochemical analyses exhibited the reduction and increase of systemic follicle-stimulating hormone (FSH) and E2 respectively after MenSCs transplantation compared to the POF rats (P < .05). No significant differences in anti-mullerian hormone (AMH) blood levels were detected in this study between POF controls and MenSCs-treated rats. We noted moreover the transcriptional up-regulation of Smad 2, 4, and TGF-β1 in POF rats, and these values were decreased after MenSCs transplantation (P < .01). By contrast, the RNA expression of Smad 6 remained increased in both pre- and post-treatment with MenSCs groups (P < .05). Finally, we found an increase in neonate births in POF rats treated with MenSCs, and that this feature was associated with ovarian rejuvenation through amelioration of fibrosis. These data showed that MenSCs are promising cell lineage for the alleviation of POF in the rat model by controlling the fibrosis rate.

中文翻译：

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经血CD146+间充质干细胞降低卵巢早衰大鼠模型纤维化率

在这里，在大鼠 POF 模型中检查了月经血来源间充质干细胞 (MenSCs) 的再生潜力，用于恢复卵巢早衰 (POF) 卵巢。新鲜分离的 CD146 ⁺将使用磁激活细胞储存方法的 MenSCs 立即注射到 POF 大鼠的卵巢中。细胞给药后 4 周和 8 周，对两个卵巢组织取样进行组织学检查和纤维化相关基因的表达。还制备了血清样品用于激素分析。在终点，进行交配试验以评估 MenSC 移植后 POF 大鼠的生育能力。组织病理学检查显示注射头孢曲松后通过增加闭锁卵泡和异常形态诱导 POF。MenSCs 移植增加了正常卵泡的数量，同时减少了卵泡闭锁。P  < .05）。在本研究中，POF 对照和 MenSCs 治疗的大鼠之间没有检测到抗苗勒管激素 (AMH) 血液水平的显着差异。我们还注意到 POF 大鼠中 Smad 2、4 和 TGF-β1 的转录上调，这些值在 MenSCs 移植后降低 ( P  < .01)。相比之下，Smad 6 的 RNA 表达在 MenSCs 组治疗前和治疗后均保持增加 ( P  < .05)。最后，我们发现用 MenSCs 治疗的 POF 大鼠的新生儿出生率增加，并且该特征与通过改善纤维化实现卵巢年轻化有关。这些数据表明，MenSCs 是通过控制纤维化率来缓解大鼠模型中 POF 的有希望的细胞谱系。