Maternal lipopolysaccharide (LPS) exposure during pregnancy induces metabolic abnormalities in male offspring, but the underlying mechanisms remain unclear. The purpose of this study was to investigate the effects of maternal LPS exposure during pregnancy on metabolic profiling of maternal serum and male fetal liver using Liquid Chromatograph Mass Spectrometer techniques. From day 15 to day 17 of gestation, pregnant mice were administered intraperitoneal LPS (experimental group) (50 μg/kg/d) or saline (control group). On day 18 of gestation, maternal serum and male fetal liver were collected. After LPS exposure, levels of 38 and 75 metabolites, mainly glycerophospholipid and fatty acid metabolites, were altered in maternal serum and male fetal liver, respectively. It was found that in maternal serum and male fetal livers, the glycerophospholipids containing saturated fatty acids (SFAs) and the SFAs were upregulated, while the glycerophospholipids containing polyunsaturated fatty acids (PUFAs) and the PUFAs were downregulated. This concordance between maternal and fetal alterations in glycerophospholipid and fatty acid metabolites may be a metabolomic signature of the early intrauterine period and may provide insight into the mechanisms by which maternal LPS exposure induces disorders of glucose metabolism in male offspring.

中文翻译：

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母体脂多糖暴露诱导的雄性后代葡萄糖代谢紊乱的母体和胎儿代谢组学改变

怀孕期间母体脂多糖 (LPS) 暴露会诱导雄性后代的代谢异常，但其潜在机制仍不清楚。本研究的目的是使用液相色谱质谱仪技术研究怀孕期间母体 LPS 暴露对母体血清和男性胎儿肝脏代谢谱的影响。从妊娠第15天到第17天，给妊娠小鼠腹腔注射LPS（实验组）（50 μg/kg/d）或生理盐水（对照组）。妊娠第18天采集母体血清和雄性胎肝。LPS 暴露后，母体血清和男性胎儿肝脏中 38 和 75 种代谢物（主要是甘油磷脂和脂肪酸代谢物）的水平分别发生了变化。发现在母体血清和男性胎儿肝脏中，含有饱和脂肪酸（SFAs）的甘油磷脂和SFAs上调，而含有多不饱和脂肪酸（PUFAs）的甘油磷脂和PUFAs下调。母体和胎儿甘油磷脂和脂肪酸代谢物变化之间的这种一致性可能是早期宫内期的代谢组学特征，并且可以提供对母体 LPS 暴露诱导雄性后代葡萄糖代谢障碍的机制的深入了解。