Water manganese (Mn) is apparently insufficient to meet aquatic animals’ metabolic requirement, and their food is considered as the major Mn source. Currently, researches reporting the effects of dietary Mn inclusion on physiological and metabolic function in Litopenaeus vannamei (L. vannamei) are very scarce. Therefore, 8-week feeding trail was conducted to determine dietary Mn requirement for L. vannamei and further evaluate the effects of dietary Mn supplementation on tissue Mn concentration, antioxidant enzyme activity, nonspecific immune enzymes activity and intestinal microbiota composition. Our results demonstrated that the optimum dietary Mn requirement for L. vannamei was determined to be 44.83 mg/kg based on the per cent weight gain; supplementing 39.57 mg/kg, 54.57 mg/kg and 72.67 mg/kg Mn significantly increased (p < .05) the activity of serum lysozyme (LZM), nitric oxide synthase and alkaline phosphatase, the activity of antioxidative capacity and glutathione in serum and hepatopancreas of L. vannamei. Supplementing 111.97 mg/kg Mn significantly increased (p < .05) hepatopancreas Mn concentration and serum LZM activity. Intestinal microbiota analysis showed that supplementing 39.57 mg/kg and 111.97 mg/kg Mn significantly influenced (p < .05) intestinal microbiota composition and function. These findings might help provide a scientific basis for the commercial application of Mn in L. vannamei.

中文翻译：

---

日粮补锰对南美白对虾生长性能、抗氧化能力、免疫功能及肠道菌群的影响

水锰（Mn）显然不足以满足水生动物的代谢需求，它们的食物被认为是主要的锰来源。目前，关于日粮添加锰对南美白对虾（L. vannamei） 生理和代谢功能影响的研究报道非常少。因此，进行了为期 8 周的饲喂试验以确定L的日粮 Mn 需求量。 vannamei并进一步评估膳食锰补充剂对组织锰浓度、抗氧化酶活性、非特异性免疫酶活性和肠道微生物群组成的影响。我们的结果表明， L的最佳日粮锰需求量。 根据体重增加百分比确定南美白为 44.83 mg/kg ；补充 39.57 mg/kg、54.57 mg/kg 和 72.67 mg/kg Mn 显着增加（p < .05）血清溶菌酶（LZM）、一氧化氮合酶和碱性磷酸酶的活性、血清中的抗氧化能力和谷胱甘肽的活性和L . 肝胰腺 南美白 补充 111.97 mg/kg Mn 显着增加 (p < .05) 肝胰腺 Mn 浓度和血清 LZM 活性。肠道菌群分析表明，补充 39.57 mg/kg 和 111.97 mg/kg Mn 显着影响（p < .05）肠道菌群组成和功能。这些发现可能有助于为锰在L中的商业应用提供科学依据。 南美白