Correlative light and electron microscopy is a valuable tool to image samples across resolution scales and link data on structure and function. While studies using this technique have been available since the 1960s, recent developments have enabled applying these workflows to large volumes of cells and tissues. Much of the development in this area has been facilitated through the collaborative efforts of microscopists and commercial companies to bring the methods, hardware and image processing technologies needed into laboratories and core imaging facilities. This is a prime example of how what was once a niche area can be brought into the mainstream of microscopy by the efforts of imaging pioneers who push the boundaries of possibility.

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相关光和体积电子显微镜 (vCLEM)：社区参与如何推动技术发展

相关光学和电子显微镜是一种有价值的工具，可以在分辨率范围内对样品进行成像，并将结构和功能的数据联系起来。虽然使用这种技术的研究自 1960 年代以来就已经可用，但最近的发展使这些工作流程能够应用于大量细胞和组织。通过显微镜专家和商业公司的合作，将所需的方法、硬件和图像处理技术带入实验室和核心成像设施，促进了该领域的大部分发展。这是一个很好的例子，说明曾经是一个小众领域如何通过推动可能性边界的成像先驱者的努力进入显微镜的主流。