Microchimica Acta ( IF 5.3 ) Pub Date : 2021-09-02 , DOI: 10.1007/s00604-021-04982-z Shan Huang 1 , Jiandong Yao 1 , Bo Li 1 , Gan Ning 1 , Qi Xiao 1
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A facile, economic, and portable test kit based on target-responsive hydrogel with smartphone detection was fabricated for the accurate determination of dichlorvos in tap water and food samples. Carbon dots (CDs) and CdTe quantum dots (QDs) embedded hydrogel were employed as indicator, and fluorescence of CdTe QDs (645 nm) was dynamically quenched by Cu2+ while that of CDs (490 nm) were non-response for Cu2+, em erging a typical ratiometric fluorescence signal. Acetylcholinesterase hydrolyzed acetylthiocholine to generate thiocholine that bound with Cu2+ strongly via S-Cu-S bond. Dichlorvos as competitive inhibitor for acetylcholinesterase prevented the generation of thiocholine, which blocked the formation of Cu-thiocholine complex and changed the ratiometric fluorescence signal. The signal of the test kit, which was recorded by smartphone’s camera, was transduced by ImageJ software into the color parameter that was linearly proportional to the logarithm of dichlorvos concentration. This portable test kit showed wide linear range of 1 to 40 ppb and low detection limit of 0.38 ppb for dichlorvos. This test kit exhibited rapid sample-to-answer detection time (50 min) of dichlorvos in tap water and food samples, and the recoveries were in the range 81.3 to 111% with relative standard deviations of less than 9.1%.
Graphical abstract
中文翻译：
将目标响应的 CD-CdTe QD 比率荧光水凝胶与智能手机相结合，用于目测和现场测定敌敌畏
基于目标响应水凝胶和智能手机检测，制作了一种简便、经济、便携的检测试剂盒，用于准确测定自来水和食品样品中敌敌畏的含量。以嵌入水凝胶的碳点（CDs）和CdTe量子点（QDs）为指示剂，CdTe QDs（645 nm）的荧光被Cu 2+动态猝灭，而CDs（490 nm）的荧光对Cu 2无响应+，出现典型的比率荧光信号。乙酰胆碱酯酶水解乙酰硫代胆碱生成与 Cu 2+结合的硫代胆碱强烈通过 S-Cu-S 键。敌敌畏作为乙酰胆碱酯酶的竞争性抑制剂阻止了硫代胆碱的产生，从而阻止了 Cu-硫代胆碱复合物的形成并改变了比率荧光信号。由智能手机摄像头记录的检测试剂盒信号，通过ImageJ软件转换成与敌敌畏浓度对数成线性正比的颜色参数。该便携式检测试剂盒对敌敌畏显示出 1 至 40 ppb 的宽线性范围和 0.38 ppb 的低检测限。该检测试剂盒对自来水和食品样品中敌敌畏的快速检测时间（50 分钟），回收率在 81.3% 至 111% 之间，相对标准偏差小于 9.1%。
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