Monitoring the effects of chemical stimuli on live cells with metasurface-enhanced infrared reflection spectroscopy,Lab on a Chip

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Monitoring the effects of chemical stimuli on live cells with metasurface-enhanced infrared reflection spectroscopy
Lab on a Chip ( IF 6.1 ) Pub Date : 2021-08-24 , DOI: 10.1039/d1lc00580d
Steven H Huang ₁ , Jiaruo Li ₁ , Zhiyuan Fan ₁ , Robert Delgado ₁ , Gennady Shvets ₁

Affiliation

Infrared spectroscopy has found wide applications in the analysis of biological materials. A more recent development is the use of engineered nanostructures – plasmonic metasurfaces – as substrates for metasurface-enhanced infrared reflection spectroscopy (MEIRS). Here, we demonstrate that strong field enhancement from plasmonic metasurfaces enables the use of MEIRS as a highly informative analytic technique for real-time monitoring of cells. By exposing live cells cultured on a plasmonic metasurface to chemical compounds, we show that MEIRS can be used as a label-free phenotypic assay for detecting multiple cellular responses to external stimuli: changes in cell morphology, adhesion, and lipid composition of the cellular membrane, as well as intracellular signaling. Using a focal plane array detection system, we show that MEIRS also enables spectro-chemical imaging at the single-cell level. The described metasurface-based all-optical sensor opens the way to a scalable, high-throughput spectroscopic assay for live cells.

中文翻译：

使用超表面增强红外反射光谱监测化学刺激对活细胞的影响

红外光谱在生物材料分析中有着广泛的应用。最近的一项发展是使用工程纳米结构（等离子体超表面）作为超表面增强红外反射光谱（MEIRS）的基底。在这里，我们证明了等离子体超表面的强场增强使得 MEIRS 能够用作实时监测细胞的信息丰富的分析技术。通过将在等离子体超表面上培养的活细胞暴露于化合物中，我们证明 MEIRS 可用作无标记表型测定，用于检测多种细胞对外部刺激的反应：细胞形态、粘附和细胞膜脂质组成的变化，以及细胞内信号传导。使用焦平面阵列检测系统，我们证明 MEIRS 还能够在单细胞水平上进行光谱化学成像。所描述的基于超表面的全光学传感器为活细胞的可扩展、高通量光谱测定开辟了道路。

更新日期：2021-09-03

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