Increasing concerns on pesticide residues in different matrices have promoted the development of various analytical techniques. Benefiting from highly-specific recognition elements and diverse signal transduction technologies, optical lateral flow test strip (LFTS) biosensors have realized the ideal candidates. They integrated the high sensitivity and stability of optical monitoring and the unique simplicity, portability, low cost, and user-friendliness of LFTS assay. The past few decades have witnessed the rapid advances of optical LFTS biosensors to meet the market and social demand for pesticide detection. Therefore, this review first summarizes the architecture and detection principle of the emerging optical LFTS biosensors for single-target inspection and multiplexed detection of pesticides through the elaborate design of different proof-of-concept types. Then, from the perspective of the design strategies and practical requirements, the primary emphasis is concentrated on the recent advances of proposed optical LFTS biosensors for pesticide detection over the past five years. A special highlight was made on colorimetric, fluorescent, chemiluminescent, and surface-enhanced Raman spectroscopy sensors through several representative state-of-the-art examples. Beside a comprehensive discussion of the advantages of these emerging optical LFTS biosensors in real application for pesticide monitoring, their potential limitations, together with current challenges and future trends are critically summarized. It is expected to afford a clear understanding and deep inspiration to researchers regarding the rapid development and practical application of more optical LFTS biosensors in analytical chemistry, food inspection, and environmental monitoring, etc.

对不同基质中农药残留的日益关注促进了各种分析技术的发展。受益于高度特异性的识别元件和多样化的信号转导技术，光学侧流测试条 (LFTS) 生物传感器已经实现了理想的候选者。他们将光学监测的高灵敏度和稳定性与 LFTS 分析独特的简单性、便携性、低成本和用户友好性相结合。过去几十年见证了光学 LFTS 生物传感器的快速发展，以满足市场和社会对农药检测的需求。所以，本综述首先通过对不同概念验证类型的精心设计，总结了新兴的用于农药单目标检测和多重检测的光学 LFTS 生物传感器的架构和检测原理。然后，从设计策略和实际要求的角度来看，主要重点集中在过去五年中提出的用于农药检测的光学 LFTS 生物传感器的最新进展。通过几个具有代表性的最先进的例子，特别强调了比色、荧光、化学发光和表面增强拉曼光谱传感器。除了全面讨论这些新兴的光学 LFTS 生物传感器在农药监测的实际应用中的优势之外，它们的潜在局限性，连同当前的挑战和未来的趋势一起进行了批判性总结。更多的光学LFTS生物传感器在分析化学、食品检验和环境监测中的快速发展和实际应用，有望为研究人员提供一个清晰的认识和深刻的启发，等。