A novel modified electrode has been successfully constructed using nanocomposites consisting of nickel oxide (NiO) and molybdenum disulfide (MoS₂) immobilized on the surface of a screen-printed carbon electrode (SPCE) for non-enzymatic cholesterol detection. Poly(methyl orange) was also immobilized on this electrode surface to act as the cholesterol-identifying agent. The electrochemical activity of the modified electrode toward cholesterol through differential pulse voltammetry showed a linear response (r² = 0.9998) in the cholesterol concentration ranges from 1 to 15 mg/dL with a limit of detection of 0.24 mg/dL, a limit of quantification of 0.81 mg/dL and sensitivity of 7.95 × 10⁻⁶ A mg⁻¹ dL⁻¹ cm⁻². Moreover, this sensor was successfully applied for the quantitative analysis of cholesterol in milk and yogurt samples. Validation using gas chromatography showed an excellent similarity value of 95.2–97.1%.

使用由固定在丝网印刷碳电极 (SPCE) 表面上的氧化镍 (NiO) 和二硫化钼 (MoS ₂ )组成的纳米复合材料成功构建了一种新型修饰电极，用于非酶促胆固醇检测。聚（甲基橙）也固定在该电极表面上，作为胆固醇识别剂。通过差分脉冲伏安法修饰电极对胆固醇的电化学活性 在胆固醇浓度范围为 1 至 15 mg/dL 时显示出线性响应 (r ² = 0.9998)，检测限为 0.24 mg/dL，定量限0.81 mg/dL，灵敏度为 7.95 × 10 ^-6  A mg ^-1  dL ^-1  cm^－2．此外，该传感器已成功应用于牛奶和酸奶样品中胆固醇的定量分析。使用气相色谱法进行的验证显示了 95.2–97.1% 的出色相似度值。