The study of the effect of cryopreservation on the functionality of monocyte-derived dendritic cells (MDDCs) and dendritic cells (DCs) is essential for their use in different clinical applications such as DCs-based vaccines. Its full maturation and its optimal functionality are crucial for DCs based immunotherapy. In this study, we compared MDDCs derived from fresh and cryopreserved PBMCs in the aspects of phenotype and its effect on T cells at the level of proliferation and cytokine secretion. We pulsed MDDCs obtained from fresh and cryopreserved PBMCs with two different stimuli, CEF and SEA, and the expression maturation markers and cytokine secretion were analyzed. Our results showed that the cryopreservation had no effects in the phenotype of the MDDCs obtained, cell viability, maturation markers expression and/or cytokines secretion, independently whether MDDCs had been generated from fresh or cryopreserved PBMCs. Thus, this study suggests that the use of cryopreserved cells is a good method to keep the cells before use in immunotherapy, avoiding the variability within same individual due to severe blood draws. Even so, the interpretation and comparison of different results should be done considering the different cryopreservation techniques and assays, and their effects on PBMCs, specifically on MDDC and DC cells.

研究冷冻保存对单核细胞衍生树突细胞 (MDDC) 和树突细胞 (DC) 功能的影响对于它们在不同临床应用中的使用至关重要，例如基于 DC 的疫苗。它的完全成熟和最佳功能对于基于 DC 的免疫治疗至关重要。在这项研究中，我们比较了来自新鲜和冷冻保存的 PBMC 的 MDDCs 在表型方面及其在增殖和细胞因子分泌水平上对 T 细胞的影响。我们用两种不同的刺激物 CEF 和 SEA 对从新鲜和冷冻保存的 PBMC 中获得的 MDDC 进行脉冲处理，并分析了表达成熟标志物和细胞因子的分泌。我们的结果表明，冷冻保存对获得的 MDDC 的表型、细胞活力、成熟标志物表达和/或细胞因子分泌没有影响，MDDCs 是由新鲜的还是冷冻保存的 PBMCs 产生的。因此，这项研究表明，使用冷冻保存的细胞是一种很好的方法，可以在用于免疫治疗之前保留细胞，避免由于严重抽血而导致同一个体内的变异性。即便如此，应考虑到不同的冷冻保存技术和分析，以及它们对 PBMC，特别是对 MDDC 和 DC 细胞的影响，对不同结果进行解释和比较。