Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Thermo-responsive fluorinated surfactant for on-demand demulsification of microfluidic droplets
Lab on a Chip ( IF 6.1 ) Pub Date : 2021-08-25 , DOI: 10.1039/d1lc00450f Chuanfeng An 1 , Yujie Zhang 1 , Hanting Li 1 , Haoyue Zhang 1 , Yonghao Zhang 1 , Jiamian Wang 2 , Yang Zhang 3 , Fang Cheng 1 , Kai Sun 1 , Huanan Wang 1
Lab on a Chip ( IF 6.1 ) Pub Date : 2021-08-25 , DOI: 10.1039/d1lc00450f Chuanfeng An 1 , Yujie Zhang 1 , Hanting Li 1 , Haoyue Zhang 1 , Yonghao Zhang 1 , Jiamian Wang 2 , Yang Zhang 3 , Fang Cheng 1 , Kai Sun 1 , Huanan Wang 1
Affiliation
|
Droplet microfluidics has recently emerged as a powerful platform for a variety of biomedical applications including microreactors, bioactive compound encapsulation, and single cell culture and analysis; all these applications require long-term droplet stability, which, however, makes breaking the emulsion and retrieving the loaded samples difficult. Herein, we developed a novel class of thermo-responsive fluorosurfactants to control the droplet status simply by temperature. The surfactants were synthesized by coupling perfluorinated polyethers (PFPEs) with a thermo-responsive block of poly(N-isopropylacrylamide) (pNIPAM) or poly(2-ethyl-2-oxazoline) (pEtOx) with lower critical solution temperature (LCST). These diblock surfactants can stabilize the emulsion at temperatures below LCST due to the hydrophilic head, which became hydrophobic upon increasing the ambient temperature above LCST, thereby destabilizing the droplets and realizing demulsification simply via temperature control. The diblock surfactant can be applied for templating cell encapsulation using alginate microgels, which allowed one-step and high-throughput microfluidic generation of cell-laden microgels without compromising cell viability. This non-invasive, on-demand demulsification strategy provides a high degree of freedom for microencapsulation and on-demand recovery of the samples or reaction products within the droplets, which opens a new avenue for a wide range of applications of droplet-templating microfluidics.
中文翻译：
用于微流体液滴按需破乳的热响应性氟化表面活性剂
液滴微流体最近已成为各种生物医学应用的强大平台，包括微反应器、生物活性化合物封装以及单细胞培养和分析；所有这些应用都需要长期的液滴稳定性，然而，这使得破乳和取回加载的样品变得困难。在此，我们开发了一类新型的热响应性含氟表面活性剂，可仅通过温度控制液滴状态。表面活性剂是通过将全氟聚醚 (PFPEs) 与聚 ( N-异丙基丙烯酰胺）（pNIPAM）或聚（2-乙基-2-恶唑啉）（pEtOx）具有较低的临界溶解温度（LCST）。这些二嵌段的表面活性剂可以稳定在低于LCST的乳液由于亲水头，这成为疏水性增加时LCST以上的环境温度，从而不稳定的液滴和简单地实现破乳经由温度控制。二嵌段表面活性剂可用于使用藻酸盐微凝胶进行细胞封装模板化，这允许一步和高通量微流体生成载有细胞的微凝胶，而不会影响细胞活力。这种非侵入性的按需破乳策略为液滴内样品或反应产物的微囊化和按需回收提供了高度的自由度，为液滴模板微流体的广泛应用开辟了新途径。
更新日期：2021-09-02
中文翻译：
用于微流体液滴按需破乳的热响应性氟化表面活性剂
液滴微流体最近已成为各种生物医学应用的强大平台，包括微反应器、生物活性化合物封装以及单细胞培养和分析；所有这些应用都需要长期的液滴稳定性，然而，这使得破乳和取回加载的样品变得困难。在此，我们开发了一类新型的热响应性含氟表面活性剂，可仅通过温度控制液滴状态。表面活性剂是通过将全氟聚醚 (PFPEs) 与聚 ( N-异丙基丙烯酰胺）（pNIPAM）或聚（2-乙基-2-恶唑啉）（pEtOx）具有较低的临界溶解温度（LCST）。这些二嵌段的表面活性剂可以稳定在低于LCST的乳液由于亲水头，这成为疏水性增加时LCST以上的环境温度，从而不稳定的液滴和简单地实现破乳经由温度控制。二嵌段表面活性剂可用于使用藻酸盐微凝胶进行细胞封装模板化，这允许一步和高通量微流体生成载有细胞的微凝胶，而不会影响细胞活力。这种非侵入性的按需破乳策略为液滴内样品或反应产物的微囊化和按需回收提供了高度的自由度，为液滴模板微流体的广泛应用开辟了新途径。
京公网安备 11010802027423号