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Simultaneous determination of three antituberculosis drugs in the serum of patients with spinal tuberculosis by capillary electrophoresis
Analytical Methods ( IF 2.7 ) Pub Date : 2021-09-02 , DOI: 10.1039/d1ay00711d Xuehua Wu 1 , Xiaoming Che 2 , Zhixue Qiu 2 , Jingyuan Chao 3 , Zhanping Kong 2 , Haili Li 4
Analytical Methods ( IF 2.7 ) Pub Date : 2021-09-02 , DOI: 10.1039/d1ay00711d Xuehua Wu 1 , Xiaoming Che 2 , Zhixue Qiu 2 , Jingyuan Chao 3 , Zhanping Kong 2 , Haili Li 4
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The pharmacokinetic variations of a single drug in an antituberculosis regimen are associated with acquired drug resistance and therapy failure. This study aimed to develop a simple and effective method for monitoring the serum levels of isoniazid (INH), rifampicin (RFP), and pyrazinamide (PZA), three antibiotics used in patients with spinal tuberculosis using capillary electrophoresis (CE). A standard solution of INH, RFP, and PZA was prepared and mixed with serum to prepare the standard curve. The detection limit, quantification limit, precision, stability, repeatability, and sample recovery were determined. Then, INH, RFP, and PZA were measured from the leftover serum samples of all patients with spinal tuberculosis who were treated with 2SHRZ/2.5H2R2Z2 combined with surgery in a tertiary hospital in Qinghai from October 2015 to September 2017. A total of 107 patients with spinal tuberculosis treated using the 2SHRZ/2.5H2R2Z2 regimen combined with surgery were included in this study. All three antibiotics had linear standard curves with high correlation coefficients (R2 = 0.9997, 0.9994, and 0.9986). The recovery rates were 98.1% for INH, 96.5% for PZA, and 97.2% for RFP. The results from the serum samples showed that the plasma concentrations of INH (4.989 ± 1.692 μg mL−1) and RFP (9.400 ± 1.711 μg mL−1) reached effective therapeutic concentrations in all patients, but not PZA (33.860 ± 1.830 μg mL−1). The CE method for measuring INH, RFP, and PZA simultaneously in serum samples of patients with spinal tuberculosis is simple, rapid, and sensitive. This method is suitable for the routine monitoring of INH, RFP, and PZA concentrations in the serum of patients with spinal tuberculosis.
中文翻译：
毛细管电泳法同时测定脊柱结核患者血清中三种抗结核药物
抗结核治疗方案中单一药物的药代动力学变化与获得性耐药和治疗失败有关。本研究旨在开发一种简单有效的方法，用于使用毛细管电泳 (CE) 监测用于脊柱结核患者的三种抗生素异烟肼 (INH)、利福平 (RFP) 和吡嗪酰胺 (PZA) 的血清水平。配制INH、RFP、PZA标准溶液，与血清混合制备标准曲线。测定了检测限、定量限、精密度、稳定性、重复性和样品回收率。然后，对2015年10月至2017年9月在青海省某三级医院接受2SHRZ/2.5H2R2Z2联合手术治疗的所有脊柱结核患者的剩余血清样本进行INH、RFP和PZA测定。本研究共纳入107例采用2SHRZ/2.5H2R2Z2方案联合手术治疗的脊柱结核患者。三种抗生素均具有线性标准曲线，相关系数高（R 2 = 0.9997、0.9994 和 0.9986）。INH 的回收率为 98.1%，PZA 的回收率为 96.5%，RFP 的回收率为 97.2%。血清样本的结果显示 INH (4.989 ± 1.692 μg mL -1 ) 和 RFP (9.400 ± 1.711 μg mL -1 ) 的血浆浓度在所有患者中均达到有效治疗浓度，但 PZA (33.860 ± 1.830 μg mL -1 ) 未达到有效治疗浓度-1 )。同时测量脊柱结核患者血清样本中 INH、RFP 和 PZA 的 CE 方法简单、快速且灵敏。该方法适用于脊柱结核患者血清中INH、RFP、PZA浓度的常规监测。
更新日期：2021-09-02
中文翻译：
毛细管电泳法同时测定脊柱结核患者血清中三种抗结核药物
抗结核治疗方案中单一药物的药代动力学变化与获得性耐药和治疗失败有关。本研究旨在开发一种简单有效的方法，用于使用毛细管电泳 (CE) 监测用于脊柱结核患者的三种抗生素异烟肼 (INH)、利福平 (RFP) 和吡嗪酰胺 (PZA) 的血清水平。配制INH、RFP、PZA标准溶液，与血清混合制备标准曲线。测定了检测限、定量限、精密度、稳定性、重复性和样品回收率。然后，对2015年10月至2017年9月在青海省某三级医院接受2SHRZ/2.5H2R2Z2联合手术治疗的所有脊柱结核患者的剩余血清样本进行INH、RFP和PZA测定。本研究共纳入107例采用2SHRZ/2.5H2R2Z2方案联合手术治疗的脊柱结核患者。三种抗生素均具有线性标准曲线，相关系数高（R 2 = 0.9997、0.9994 和 0.9986）。INH 的回收率为 98.1%，PZA 的回收率为 96.5%，RFP 的回收率为 97.2%。血清样本的结果显示 INH (4.989 ± 1.692 μg mL -1 ) 和 RFP (9.400 ± 1.711 μg mL -1 ) 的血浆浓度在所有患者中均达到有效治疗浓度，但 PZA (33.860 ± 1.830 μg mL -1 ) 未达到有效治疗浓度-1 )。同时测量脊柱结核患者血清样本中 INH、RFP 和 PZA 的 CE 方法简单、快速且灵敏。该方法适用于脊柱结核患者血清中INH、RFP、PZA浓度的常规监测。
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