Mitochondria are dynamic organelles that differ significantly in their morphologies across cell types, reflecting specific cellular needs and stages in development. Despite the wide biological significance in disease and health, delineating mitochondrial morphologies in complex systems remains challenging. Here, we present the Mitochondrial Cellular Phenotype (MitoCellPhe) tool developed for quantifying mitochondrial morphologies and demonstrate its utility in delineating differences in mitochondrial morphologies in a human fibroblast and human induced pluripotent stem cell (hiPSC) line. MitoCellPhe generates 24 parameters, allowing for a comprehensive analysis of mitochondrial structures and importantly allows for quantification to be performed on mitochondria in images containing single cells or clusters of cells. With this tool, we were able to validate previous findings that show networks of mitochondria in healthy fibroblast cell lines and a more fragmented morphology in hiPSCs. Using images generated from control and diseased fibroblasts and hiPSCs, we also demonstrate the efficacy of the toolset in delineating differences in morphologies between healthy and the diseased state in both stem cell (hiPSC) and differentiated fibroblast cells. Our results demonstrate that MitoCellPhe enables high-throughput, sensitive, detailed and quantitative mitochondrial morphological assessment and thus enables better biological insights into mitochondrial dynamics in health and disease

中文翻译：

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MitoCellPhe 揭示单个成纤维细胞和成簇干细胞的线粒体形态


线粒体是动态细胞器，不同细胞类型的形态存在显着差异，反映了特定的细胞需求和发育阶段。尽管在疾病和健康方面具有广泛的生物学意义，但描绘复杂系统中的线粒体形态仍然具有挑战性。在这里，我们展示了为量化线粒体形态而开发的线粒体细胞表型 (MitoCellPhe) 工具，并证明了其在描绘人成纤维细胞和人诱导多能干细胞 (hiPSC) 系中线粒体形态差异方面的实用性。 MitoCellPhe 生成 24 个参数，可以对线粒体结构进行全面分析，重要的是可以在包含单细胞或细胞簇的图像中对线粒体进行定量。借助该工具，我们能够验证之前的研究结果，即健康成纤维细胞系中的线粒体网络以及 hiPSC 中更加碎片化的形态。使用从对照和患病成纤维细胞以及 hiPSC 生成的图像，我们还证明了该工具集在描绘干细胞 (hiPSC) 和分化成纤维细胞的健康状态和患病状态之间形态差异方面的功效。我们的结果表明，MitoCellPhe 能够实现高通量、灵敏、详细和定量的线粒体形态学评估，从而能够更好地了解健康和疾病中的线粒体动力学