Efficacy of different doses of the Beauveria bassiana-IRAN441C alone (66,125, 250, 500, 1000 mg conidia. Kg⁻¹ grains) and combined with a low-lethal dose (LD₂₅) of diatomaceous earth (DE) SilicoSec® as lethal agents were assessed against the adults of Callosobruchus maculatus (F.) (Chrysomelidae) on mung, and cowpeas, and Oryzaephilus surinamensis (L.) (Silvanidae) on wheat and barley seeds. In both species, mortality reached 60–73% in B. bassiana alone and 83–100% in fungus combined with the DE at the highest dose. A synergistic effect between B. bassiana conidia and the DE was confirmed. The DE low-lethal dose alters fungal mycosis and sporogenesis (conidia mL⁻¹) rates in the infected cadavers. The adult populations of both species were suppressed desirably despite a decrease in the number of cadaver-derived mycosis and their spore production at the highest combination doses (1000 mg kg⁻¹+ LD₂₅ of DE). Seed type had no significant effect on mycosis and sporogenesis on both insects in several combination doses. The cadaver-derived aerial conidia germination rate was increased in the presence of DE low-lethal dose, but it declined by increasing the fungal dose. Type of commodity failed to affect the germination rate of O. surinamensis-derived conidia; however, the mung bean intensified this case for another insect. In both beetles, offspring's emergence was suppressed dose-dependently seven weeks after removing exposed parents to the lethal agents as well it was more in doses mixed with DE. The most significant reduction in progeny production of treated C. maculatus occurred on cowpeas and that of treated O. surinamensis on wheat seeds. Our findings demonstrate that DE's low-lethal dose enhances the virulence of B. bassiana in the various mixtures synergistically, and co-applications of the two substances were feasible. As well, the lethal agents' adverse effects were found to carry over onto both species' offspring during two-month storage time.

单独使用不同剂量的白僵菌-IRAN441C（66,125、250、500、1000 毫克分生孢子。Kg ^-1粒）并与低致死剂量 (LD ₂₅ ) 的硅藻土 (DE) SilicoSec® 组合作为致死剂的功效对绿豆和豇豆上的Callosobruchus maculatus (F.) (Chrysomelidae) 和小麦和大麦种子上的Oryzaephilus surinamensis (L.) ( Silvanidae )的成虫进行了评估。在这两个物种中，仅在B. bassiana 中死亡率达到 60-73%，在最高剂量下与 DE 结合的真菌中死亡率达到 83-100% 。B. bassiana之间的协同效应分生孢子和DE得到确认。DE 低致死剂量改变了受感染尸体中的真菌真菌病和孢子发生（分生孢子 mL ^-1）率。尽管在最高组合剂量（1000 mg kg ^-1 + LD ₂₅的DE）下，尸体来源的真菌病的数量和它们的孢子产生减少，但两种物种的成年种群都被理想地抑制。在几种组合剂量下，种子类型对两种昆虫的真菌病和孢子发生没有显着影响。在DE低致死剂量存在下，尸体来源的气生分生孢子萌发率增加，但随着真菌剂量的增加而下降。商品类型未能影响O. surinamensis的发芽率-衍生的分生孢子；然而，绿豆为另一种昆虫加剧了这种情况。在这两种甲虫中，在将暴露于致死剂的亲本移除 7 周后，后代的出现受到剂量依赖性抑制，并且在与 DE 混合的剂量下也更多。处理过的C. maculatus后代产量的最显着降低发生在豇豆上，而处理过的O. surinamensis在小麦种子上的后代产量减少。我们的研究结果表明，DE 的低致死剂量可协同增强B. bassiana在各种混合物中的毒力，并且这两种物质的共同应用是可行的。同样，在两个月的储存时间内，发现致死剂的不利影响会延续到两个物种的后代身上。