In the present study, a chemosensor (TH) was developed for fluorometric detection of cholesterol. The chemosensor was synthesized by condensation reaction of 4-phenylthiazole-2-semicarbazide with 5-chlorosalicylaldehyde, and was successfully characterized by ¹H NMR, ¹³C NMR and HRMS. The compound shows absorption at 282 nm, 334 nm and 384 nm region and exhibits strong emission at 441 nm upon excitation at 374 nm. The sensing studies show significant fluorescence quenching upon addition of cholesterol to TH solution in dimethyl formamide (DMF). The self-association tendency (micelle formation) of cholesterol molecule leads to the supramolecular assemblies of TH and cholesterol in DMF solution thereby quenching the fluorescence response. The intercalation of TH into the micelle of cholesterol was further confirmed by fluorescence anisotropy study wherein the anisotropy value gets significantly enhanced upon the addition of cholesterol to the solution of TH. The developed chemosensor exhibits high selectivity for cholesterol with the linear range of 66–830 µM and a limit of detection (LOD) of 26 µM.

在本研究中，开发了一种化学传感器 ( TH ) 用于胆固醇的荧光检测。该化学传感器由4-苯基噻唑-2-氨基脲与5-氯水杨醛缩合反应合成，并通过¹ H NMR、¹³ C NMR和HRMS成功表征。该化合物在 282 nm、334 nm 和 384 nm 区域显示吸收，并在 374 nm 激发时在 441 nm 显示强发射。传感研究表明，将胆固醇添加到二甲基甲酰胺 (DMF) 中的TH溶液后，荧光会显着猝灭。胆固醇分子的自缔合倾向（胶束形成）导致TH的超分子组装和胆固醇在 DMF 溶液中，从而淬灭荧光反应。荧光各向异性研究进一步证实了TH嵌入胆固醇胶束中，其中向TH溶液中加入胆固醇后各向异性值显着增强。开发的化学传感器对胆固醇具有高选择性，线性范围为 66–830 µM，检测限 (LOD) 为 26 µM。