In situ microplates are small in size, crystal cultivation and operation are difficult, and the efficiency of crystal screening is relatively low. To solve this problem, a novel combined crystallization plate was designed for high-throughput crystal cultivation and in situ data collection. A frame was used to hold 48 in situ microplates, and the in situ microplates were sealed on one side with an ultralow background-scattering Kapton film. An automatic liquid handler (Mosquito) was used to add a liquid drop to the in situ microplates in the frame, and CrystalClear HD tape was used to seal the frame. A sealed frame holding 48 microplates was developed as a novel combined crystallization plate and was used for crystal cultivation under different conditions and in situ data collection at the synchrotron beamline. Moreover, individual microplates can be separated from the combined crystal plate and then fixed on a magnetic base or loaded onto a UniPuck for in situ data collection. Automatic grid scanning was used to locate crystals. The efficiency of the combined crystallization plate for crystal screening was verified. This method avoids the manual manipulation of crystals during crystal screening and diffraction data collection; therefore, the combined crystallization plate is suitable for large-scale screening of microcrystals.

中文翻译：

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用于高通量晶体筛选和晶体学光束线原位数据收集的新型组合结晶板

原位微孔板体积小，晶体培养和操作困难，晶体筛选效率较低。为了解决这个问题，设计了一种新型组合结晶板，用于高通量晶体培养和原位数据收集。一个框架用于固定 48 个原位微板，并且原位微板的一侧用超低背景散射 Kapton 薄膜密封。使用自动液体处理器（Mosquito）将液滴添加到框架中的原位微孔板中，并使用 CrystalClear HD 胶带密封框架。开发了一种容纳 48 个微板的密封框架作为新型组合结晶板，用于不同条件下的晶体培养和同步加速器光束线的原位数据收集。此外，单个微板可以从组合晶体板中分离出来，然后固定在磁性底座上或加载到 UniPuck 上进行原位数据收集。使用自动网格扫描来定位晶体。验证了组合结晶板用于晶体筛选的效率。该方法避免了晶体筛选和衍射数据收集过程中对晶体的手动操作；因此，组合结晶板适合微晶的大规模筛选。