In this study, we aimed to detect the clinical significance and potential mechanism of LINC00888 in laryngeal cancer. Data from The Cancer Genome Atlas (TCGA) afforded the expression of LINC00888 in laryngeal cancer samples. The clinical significance of LINC00888 expression in laryngeal cancer was demonstrated by χ², Cox analysis, and Kaplan–Meier. The downstream targets of LINC00888 were identified based on analysis from bioinformatics and laryngeal cancer-related TCGA data sets. The microRNA-378g (miR-378g)/TFRC (transferrin receptor) axis was selected and identified by qRT-PCR, Western blot analysis, and luciferase activity assays. Cell counting kit-8, colony formation, and transwell assays were applied to detect the phenotypes of laryngeal cancer cells. We observed that LINC00888 expression was notably increased in laryngeal cancer and associated with death, recurrence, and prognosis. Depletion of LINC00888 repressed the proliferative and motile abilities of laryngeal cancer cells in vitro. LINC00888 was predicted to act as a competing endogenous RNA within the microRNA (miRNA)/messenger RNA (mRNA) axis based on analysis from bioinformatics and laryngeal cancer-related TCGA data sets. Interestingly, we discovered that LINC00888 functioned as an miRNA sponge to suppress the effect of miR-378g on laryngeal cancer cells behaviors, as well as positively regulate TFRC expression. Furthermore, the knockdown of TFRC strengthened the inhibitory effect of si-LINC00888 on laryngeal cancer cells' malignant properties. LINC00888 is an oncogenic lncRNA that promotes the growth and mobility of laryngeal cancer cells by controlling laryngeal cancer-related mRNA and tumor-suppressive miRNA. The LINC00888/miR-378g/TFRC pathway might lead to the development of laryngeal cancer cells and, therefore, might be a candidate therapeutic target for laryngeal cancer.

中文翻译：

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LncRNA LINC00888 上调预测喉癌患者的生存期较差，并通过调节 miR-378g/TFRC 加速喉癌细胞的生长和迁移

本研究旨在探讨LINC00888在喉癌中的临床意义和潜在机制。来自癌症基因组图谱 (TCGA) 的数据提供了 LINC00888 在喉癌样本中的表达。LINC00888在喉癌中表达的临床意义由χ ^2证明, Cox 分析和 Kaplan-Meier。LINC00888 的下游靶点是根据生物信息学和喉癌相关 TCGA 数据集的分析确定的。通过 qRT-PCR、蛋白质印迹分析和荧光素酶活性测定选择和鉴定 microRNA-378g (miR-378g)/TFRC（转铁蛋白受体）轴。应用细胞计数 kit-8、集落形成和 transwell 测定来检测喉癌细胞的表型。我们观察到 LINC00888 表达在喉癌中显着增加，并与死亡、复发和预后相关。LINC00888 的消耗抑制了体外喉癌细胞的增殖和运动能力。根据对生物信息学和喉癌相关 TCGA 数据集的分析，预测 LINC00888 在 microRNA (miRNA)/信使 RNA (mRNA) 轴内充当竞争性内源性 RNA。有趣的是，我们发现 LINC00888 作为 miRNA 海绵发挥作用，可抑制 miR-378g 对喉癌细胞行为的影响，并正向调节 TFRC 表达。此外，TFRC的敲低增强了si-LINC00888对喉癌细胞恶性特性的抑制作用。LINC00888 是一种致癌 lncRNA，通过控制喉癌相关 mRNA 和抑癌 miRNA 来促进喉癌细胞的生长和迁移。LINC00888/miR-378g/TFRC 通路可能导致喉癌细胞的发展，因此，