当前位置: X-MOL 学术Chirality › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Bovine serum albumin-coated titanium dioxide modified electrochemical interface for enantioselective discrimination of D/L-aspartic acid
Chirality ( IF 2.8 ) Pub Date : 2021-09-01 , DOI: 10.1002/chir.23356
Xiaofeng Wei 1, 2, 3 , Ying Chen 1 , Shan He 1 , Huiting Lian 1, 2 , Xuegong Cao 1, 2 , Bin Liu 1, 2, 3
Affiliation  

Due to the similar physicochemical properties, the discrimination of chiral isomers faces huge challenges in drug production and biochemical analysis. Herein, the bovine serum albumin-coated titanium dioxide (bovine serum albumin [BSA]/TiO2) was modified as a novel electrochemical interface for efficient, simple, and enantioselective discrimination of aspartic acid enantiomers (D/L-Asp) based on the electrochemical impedance spectroscopy (EIS). Utilizing the structural characteristics of large cavity and high specific surface area, TiO2 material provided sufficient space for adequate loading of BSA. The BSA/TiO2 electrochemical interface was successfully fabricated to support abundant chiral recognition sites. The enantioselective discrimination of D/L-Asp was achieved on the interface with a good linear relationship against the impedance difference in the concentration range from 1 to 1000 nM with the detection limit of 0.37 nM for L-Asp and 0.94 nM for D-Asp, reaching the identification coefficient (Ic = KL/KD) of 1.85. The proposed interface is easy to form with a stable formation of BSA in TiO2 microporous architecture, which maintained the desired stability and reproducibility. For the unknown racemic solution, Ic levels of different enantio-ratios of D/L-Asp were effectively obtained to evaluate the chiral percentage of racemic sample. The possible mechanism of chiral recognition by density function theory (DFT) was confirmed with a stronger adsorption to L-Asp in accordance with our experiment results, reinforcing the validity of our presented interface. The BSA/TiO2 electrochemical interface with robust enantioselective discrimination of D/L-Asp has great potential for the practical application in pharmaceutical surveillance and food security.

中文翻译:

牛血清白蛋白包覆的二氧化钛修饰电化学界面用于 D/L-天冬氨酸的对映选择性鉴别

由于物理化学性质相似,手性异构体的鉴别在药物生产和生化分析中面临巨大挑战。在此,牛血清白蛋白包被的二氧化钛(牛血清白蛋白 [BSA]/TiO 2)被修饰为一种新型电化学界面,用于基于电化学阻抗谱(EIS)。利用大空腔和高比表面积的结构特点,TiO 2材料为BSA的充分负载提供了足够的空间。BSA/TiO 2成功制造了电化学界面以支持丰富的手性识别位点。D/L-Asp 的对映选择性鉴别是在界面上实现的,在 1 到 1000 nM 的浓度范围内与阻抗差异具有良好的线性关系,L-Asp 的检测限为 0.37 nM,D-Asp 的检测限为 0.94 nM ,达到1.85的识别系数(I c  = K L /K D)。所提出的界面易于形成,在 TiO 2微孔结构中稳定形成 BSA,保持了所需的稳定性和再现性。对于未知的外消旋溶液,I c有效地获得了D/L-Asp的不同对映比水平,以评估外消旋样品的手性百分比。根据我们的实验结果,通过对 L-Asp 的更强吸附证实了密度泛函理论 (DFT) 手性识别的可能机制,增强了我们提出的界面的有效性。BSA/TiO 2电化学界面具有强大的 D/L-Asp 对映选择性鉴别能力,在药物监测和食品安全方面的实际应用具有巨大潜力。
更新日期:2021-09-17
down
wechat
bug