Premna flavescens Wall. ex C. B. Clarke, a medicinal plant in the family Lamiaceae, was used for the treatment of arthritis in folk medicine. From the essential oil extracted from the leaves of this plant we have identified 51 components, with β-caryophyllene as the major component (26.3%). The essential oil showed a strong anti-inflammatory effect in vitro with an IC₅₀ of 5.88 µg/mL. DNA barcoding is an useful tool for species identification based on the standardized genomic DNA fragments, but the molecular database of P flavescens is still lacking from Genebank. A molecular species identification tool for P flavescens was developed for the first time using DNA barcoding. The sequences of rbcL, trnH–psbA, internal transcribed spacer, and 18S barcodes of P flavescens were submitted to the Genebank with the accession numbers MW553265, MW553266, MT935698.1, and MW485128.1, respectively. This will be highly useful for the molecular authentication of the marker samples.

Premna 苦参墙。ex CB Clarke，唇形科的一种药用植物，在民间医学中用于治疗关节炎。从这种植物的叶子中提取的精油中，我们鉴定了 51 种成分，其中主要成分为β-石竹烯 (26.3%)。该精油在体外表现出很强的抗炎作用，IC ₅₀为 5.88 µg/mL。DNA 条形码是基于标准化基因组 DNA 片段进行物种鉴定的有用工具，但Genebank 仍缺乏苦参的分子数据库。苦参的分子种类鉴定工具是首次使用 DNA 条形码开发的。将苦参的rbcL、trnH-psbA、内部转录间隔区和18S 条码的序列提交给Genebank，登录号分别为MW553265、MW553266、MT935698.1 和MW485128.1。这对于标记样品的分子认证非常有用。