Huperzine A, isolated from Huperzia serrata, is a potent, specific, and reversible inhibitor of acetylcholinesterase with high efficiency and low toxicity. To evaluate the presence of huperzine A in Vietnamese H serrata, a reliable capillary zone electrophoresis method was developed. The analytical conditions were established using 80 mM ammonium acetate buffer, pH 6.0, hydrodynamic injection at 50 mbar for 5 s, applied voltage of 20 kV, temperature at 25 °C, uncoated fused-silica capillary, 56 cm (50 cm effective length) × 70 µm inner diameter, and ultraviolet detection at 310 nm. The recovery rates ranged from 98.05% to 100.64%, with a relative standard deviation <2%. Good linear regression was observed in the concentration range of 1 to 500 µg/mL, with a correlation coefficient of 0.9994. The limit of detection and limit of quantification were 0.33 and 1.0 µg/mL, respectively. These results demonstrate that this method is simple, selective, and suitable for performing quality control for huperzine A derived from Vietnamese H serrata.

从锯齿石杉中分离得到的石杉碱 A是一种强效、特异性、可逆的乙酰胆碱酯酶抑制剂，具有高效低毒的特点。为了评估石杉碱甲在越南存在^ h锯缘青蟹，开发了一种可靠的毛细管区带电泳方法。分析条件是使用 80 mM 醋酸铵缓冲液，pH 6.0，在 50 mbar 下流体动力学注射 5 秒，施加电压 20 kV，温度 25 °C，无涂层熔融石英毛细管，56 cm（50 cm 有效长度） × 70 µm 内径，紫外检测波长为 310 nm。回收率范围为 98.05% 至 100.64%，相对标准偏差 <2%。在 1 至 500 µg/mL 的浓度范围内观察到良好的线性回归，相关系数为 0.9994。检测限和定量限分别为 0.33 和 1.0 µg/mL。这些结果表明，该方法简单、选择性好，适用于对来自越南锯缘锯缘青蟹的石杉碱 A 进行质量控制。.