Background

Cystic echinococcosis (CE) is a common chronic zoonotic parasitic disease infected with the Echinococcus granulosus. This study aimed to construct the competitive endogenous RNA (ceRNA) network and investigate the mechanism of mice in echinococcosis sensitization.

Methods

The animal model of echinococcosis was established in mice, and the RNA sequencing was then performed using cysts. The differentially expressed RNAs (DERNAs: DEmRNAs, DEmiRNAs, and DElncRNAs) were screened between anaphylactic mice or non-anaphylactic mice and controls, respectively. The interactions of two DERNAs groups were identified and the ceRNA network was constructed. Moreover, the potential biological functions and pathways of the DEmRNAs were explored by enrichment analyses. Finally, the qRT-PCR and the western blot were performed to validate the expression levels of key RNAs and proteins.

Results

A total of 285 common DEmRNAs, 157 common DElncRNAs and 4 common DEmiRNAs were observed. CeRNA network contained 3 DElncRNAs, 4 DEmiRNAs, and 27 DEmRNAs corporately. Enrichment results revealed that the functions of DEmRNAs focus on biological functions and pathways that specifically interact with the immune inflammatory response. In addition, the expression of 1700099I09Rik, let-7a-5p, Ccl28 and IL-13 was validated by RT-qPCR and western blot.

Conclusion

In this study, ceRNA network associated with CE sensitization in mice was constructed. The DEmRNAs in this network may be key clues for the immune mechanism of CE sensitization.

中文翻译：

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包虫病致过敏反应小鼠ceRNA调控网络的构建

背景

囊性棘球蚴病 (CE) 是一种常见的慢性人畜共患寄生虫病，感染细粒棘球绦虫。本研究旨在构建竞争性内源RNA（ceRNA）网络，探讨小鼠对棘球蚴病致敏的机制。

方法

在小鼠体内建立包虫病动物模型，然后利用包囊进行RNA测序。分别在过敏小鼠或非过敏小鼠与对照之间筛选差异表达的 RNA（DERNA：DEmRNA、DEmiRNA 和 DElncRNA）。确定了两个 DERNA 组的相互作用并构建了 ceRNA 网络。此外，通过富集分析探索了 DEmRNA 的潜在生物学功能和途径。最后，进行 qRT-PCR 和蛋白质印迹以验证关键 RNA 和蛋白质的表达水平。

结果

共观察到 285 个常见的 DEmRNA、157 个常见的 DElncRNA 和 4 个常见的 DEmiRNA。CeRNA 网络共包含 3 个 DElncRNA、4 个 DEmiRNA 和 27 个 DEmRNA。富集结果表明，DEmRNAs 的功能集中在与免疫炎症反应特异性相互作用的生物学功能和途径上。此外，通过 RT-qPCR 和蛋白质印迹验证了 1700099I09Rik、let-7a-5p、Ccl28 和 IL-13 的表达。

结论

本研究构建了与小鼠 CE 致敏相关的 ceRNA 网络。该网络中的 DEmRNAs 可能是 CE 致敏免疫机制的关键线索。