Background

Ricinus communis L. (castor bean) is valued for its oil and the performance of oil is closely related to its fatty acid composition. Thus, producing oil in vitro with favored fatty acid profiles is a promising research area and may also offer industrial opportunities.

Material and Method

In line with this, the total amount of oil and the fatty acid composition of the samples, which were endosperm and calli obtained by treatment of various doses of plant growth regulators were determined.

Results

Results showed that the type and amount of the plant growth regulator used in the media affect the fatty acid composition. In detail, the biggest change was shown by Indole-3-Acetic Acid (IAA), in general, using the plant growth regulators at 5 mg L⁻¹, instead of 20 mg L⁻¹, was found to have induced larger differentiations. The effect of a natural plant growth regulator (IAA) on fatty acid profiles was bigger than the synthetic ones (NAA, 1-Naphthaleneacetic acid, and 2,4 D, 2,4-Dichlorophenoxyacetic acid). The media containing 5 mg L⁻¹ of NAA, 20 mg L⁻¹ of NAA, 20 mg L⁻¹ of 2,4 D, or 5 mg L⁻¹ of 2,4 D gave similar results.

中文翻译：

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植物生长物质对蓖麻油含量和脂肪酸组成影响的体外研究

 背景

蓖麻子因其油而受到重视，而油的性能与其脂肪酸组成密切相关。因此，体外生产具有有利脂肪酸谱的油是一个有前途的研究领域，也可能提供工业机会。

 材料与方法

据此，测定了通过不同剂量的植物生长调节剂处理获得的胚乳和愈伤组织样品的油总量和脂肪酸组成。

 结果

结果表明，培养基中使用的植物生长调节剂的类型和用量会影响脂肪酸组成。具体来说，最大的变化是Indole-3-Acetic Acid (IAA)，一般来说，使用5 mg L ^-1的植物生长调节剂，而不是20 mg L ^-1 ，被发现诱导了更大的分化。天然植物生长调节剂 (IAA) 对脂肪酸谱的影响大于合成植物生长调节剂（NAA、1-萘乙酸和 2,4 D,2,4-二氯苯氧基乙酸）。含有5mg L ^-1 NAA、20mg L ^-1 NAA、20mg L ^-1 2,4 D或5mg L ^-1 2,4 D的培养基给出相似的结果。