Methodologies for imposing stress and reproducible results are a bottleneck for breeding programmes, and this is due to the lack of consensus between the existing methodologies. The aim of the present study was to propose and validate a new methodology for imposing water deficit in soybean that allows the identification of water deficit-tolerant genotypes, at different harvest times and phenological stages. The methodology was based on the construction of a water retention curve in the soil to determine the water stresses that indicate the field capacity and the permanent wilt point and, thus, define the water regime in the conditions of control and stress. Seven trials were carried out to validate the methodology. In trials 1, 2, 3, 4, 5 and 6, the water deficit was imposed in the reproductive phase and the components of production were evaluated. In addition to these variables, leaf water potential was evaluated in trial 6. In trial 7, the plants were subjected to water deficit in the vegetative phase and the morphological traits were evaluated. The efficiency of the methodology was confirmed by the distinction between the conditions of control and stress, affirmed by the statistical differences in most of the traits evaluated in the reproductive and vegetative phases.

中文翻译：

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一种模拟大豆基因型水分亏缺的新方法

施加压力和可重复结果的方法是育种计划的瓶颈，这是由于现有方法之间缺乏共识。本研究的目的是提出并验证一种在大豆中施加水分亏缺的新方法，该方法允许在不同的收获时间和物候阶段鉴定耐缺水的基因型。该方法基于构建土壤中的保水曲线，以确定表明田间持水量和永久枯萎点的水分胁迫，从而确定控制和胁迫条件下的水分状况。进行了七项试验以验证该方法。在试验 1、2、3、4、5 和 6 中，在繁殖阶段强加了水分亏缺，并对生产的组成部分进行了评估。除了这些变量之外，在试验 6 中还评估了叶水势。在试验 7 中，植物在营养阶段遭受水分亏缺，并评估了形态特征。该方法的有效性通过控制和压力条件之间的区别得到证实，在生殖和营养阶段评估的大多数性状的统计差异证实了这一点。