Uncontrolled hemorrhage accounts for significant death risk both in trauma and surgery. Various bleeding control techniques have been emerged to augment hemostasis, which still has several limitations and drawbacks. In this study, epinephrine-entrapped chitosan nanoparticles were electrosprayed on a base pad and covered by a gelatin nanofiber layer (E-CS-Gl. Physico-chemical characteristics, hemocompatibility, cytotoxicity, and blood coagulation tests were studied in-vitro, and blood coagulation and hemostasis potential tests were performed in-vivo. The in-vitro results showed that the prepared nano-biomaterial is cytocompatible against HuGu cells. Also, hemocompatibility studies showed that PT and aPTT times did not change in comparison with the controls. Further blood coagulation study indicated that E-CS-Gl provides an ultimate interface to induce red blood cell absorption and aggregation, resulting in augmented blood coagulation. E-CS-Gl also caused rapid clotting in rat models of ruptured femoral artery and liver compared to controls. Findings exhibited that E-CS-Gl is a safe and effective hemostatic agent and provides a new approach for fast and safe hemorrhage control.

在外伤和手术中，不受控制的出血导致显着的死亡风险。已经出现了各种出血控制技术来增强止血，但止血仍然存在一些局限性和缺点。在这项研究中，肾上腺素包埋壳聚糖纳米颗粒电喷雾上的基垫，并通过明胶的纳米纤维层覆盖（E-CS-GL。物理化学特性，血液相容性，细胞毒性和血液凝固试验研究在体外，和血凝血和止血潜力被执行测试的体内。该体外结果表明，制备的纳米生物材料对 HuGu 细胞具有细胞相容性。此外，血液相容性研究表明，与对照组相比，PT 和 aPTT 时间没有变化。进一步的血液凝固研究表明，E-CS-G1 提供了一个最终界面来诱导红细胞吸收和聚集，从而增强血液凝固。与对照相比，E-CS-G1还在股动脉和肝脏破裂的大鼠模型中引起快速凝血。研究结果表明，E-CS-G1 是一种安全有效的止血剂，为快速安全的出血控制提供了一种新方法。