This work aimed to evaluate the phytochemicals, antioxidants, antidiabetic, and biocompatibility of methanolic extract and its various solvent fractions (hexane, ethyl acetate, methanol, methanol/water) of Gynura procumbens (GP). Among the extract/fractions, ethyl acetate fraction (EA-F) exhibited the higher total phenol content (88.52 ± 1.21 GAE μg/g) and total flavonoid content (51.69 ± 1.16 QE μg/g) with higher DPPH, ABTS radical scavenging, and enzyme inhibition activities. The IC₅₀ of GP-EA-F were 144 ± 12.1 μg/mL for DPPH scavenging, 72 ± 2 μg/mL for ABTS⁺ scavenging, 90.7 ± 2.5 μg/mL for α-glucosidase inhibition and 136 ± 4 μg/mL for α-amylase inhibition. Also, GP-EA-F augmented the glucose uptake in insulin-resistant (IR) HepG2 cells. Besides, the ultra-high-performance liquid chromatography quadrupole time of flight mass spectrometer (UPLC/QTOF-MS/MS) analysis revealed the presence of phytochemicals including kaempferol, quercetin, caffeoyl-O-hexoside, caffeoylquinic acid, coumaroyl-O-hexoside, and coumaroylquinic acid in GP-EA-F. Further, the molecular docking analysis demonstrated the strong molecular interaction between the digestive enzymes (α-glucosidase and α-amylase) and selected molecules. Hence, this work concluded that GP-EA-F contains potent antidiabetic molecules which deserved further isolation, purification, and pharmacological studies.

这项工作旨在评估Gynura procumbens (GP)的甲醇提取物及其各种溶剂级分（己烷、乙酸乙酯、甲醇、甲醇/水）的植物化学物质、抗氧化剂、抗糖尿病和生物相容性。在提取物/馏分中，乙酸乙酯馏分 (EA-F) 表现出较高的总酚含量 (88.52 ± 1.21 GAE μg/g) 和总黄酮含量 (51.69 ± 1.16 QE μg/g)，具有更高的 DPPH、ABTS 自由基清除能力、和酶抑制活性。GP-EA-F的 IC ₅₀对 DPPH 清除为 144 ± 12.1 μg/mL，对 ABTS ⁺为 72 ± 2 μg/mL清除，α-葡萄糖苷酶抑制为 90.7 ± 2.5 μg/mL，α-淀粉酶抑制为 136 ± 4 μg/mL。此外，GP-EA-F 增加了胰岛素抵抗 (IR) HepG2 细胞的葡萄糖摄取。此外，超高效液相色谱四极杆飞行时间质谱仪 (UPLC/QTOF-MS/MS) 分析揭示了植物化学物质的存在，包括山奈酚、槲皮素、咖啡酰-O-己糖苷、咖啡酰奎宁酸、香豆酰-O-己糖苷和 GP-EA-F 中的香豆酰奎宁酸。此外，分子对接分析表明消化酶（α-葡萄糖苷酶和α-淀粉酶）与选定分子之间存在强分子相互作用。因此，这项工作得出结论，GP-EA-F 含有有效的抗糖尿病分子，值得进一步分离、纯化和药理学研究。