Lignocellulose pretreatment by ionic liquid (IL) displays enormous potential in biomass conversion, however, high cost impedes its large-scale application in biorefinerry process. In this study, a strategy evolving a high substrate loading was developed to address this challenge. Rice straw (RS) was first pretreated with 1-ethyl-3-methylimidazolium acetate at a high substrate loading of 25 wt.%, and then followed by bacterial delignification. The corresponding reducing sugar and glucose yield was 510.3 and 324.5 mg/g, respectively, which increased by 1.4 and 1.37 times over those of the sole IL pretreatment. The IL pretreatment significantly changed the structure of RS and reduced the crystallinity of cellulose, and the biological pretreatment with a bacterial strain of Cupriavidus basilensis B-8 removed most of the lignin residual. Meanwhile, the cellulose was well reserved. The combination pretreatment contributed to a high yield of the fermentable sugar in an economical way.

离子液体 (IL) 预处理木质纤维素在生物质转化中显示出巨大的潜力，但高成本阻碍了其在生物炼制过程中的大规模应用。在这项研究中，开发了一种发展高基板负载的策略来应对这一挑战。稻草 (RS) 首先用 1-乙基-3-甲基咪唑鎓醋酸盐以 25 wt.% 的高底物负载量进行预处理，然后进行细菌脱木素。相应的还原糖和葡萄糖收率分别为510.3和324.5 mg/g，比单独IL预处理增加了1.4和1.37倍。IL预处理显着改变了RS的结构并降低了纤维素的结晶度，Cupriavidus basilensis菌株的生物预处理B-8 去除了大部分木质素残留物。同时，纤维素得到了很好的保留。组合预处理有助于以经济的方式高产可发酵糖。