Mesoporous silicon (PSi) modified glassy carbon electrode (GCE) was studied for the first time for efficient detection of dopamine (DA). A facile stain etching method was used to synthesize the single crystalline PSi nanoparticles, which was confirmed by the SAED. The morphological study of PSi NPs by FESEM and TEM showed the random distribution of pores with less than 25 nm pore size. The X-ray diffraction (XRD), Raman spectra, and Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) were also used in characterizing the PSi NPs. The-as fabricated PSi NPs modified GCE biosensor can measure a wide range of dopamine (0.5–333.3 μM) in phosphate buffer solution (PBS) with a sensitivity value of 0.2715 μAμM⁻¹cm⁻² with an extremely low limit of detection (LOD) of 3.2 nM. This non-enzymatic dopamine biosensor was also tested for the possible impact of common interfering substances, which showed very good selectivity. The current modified electrode was further employed to analyze human blood serums and dopamine hydrochloride injection samples to detect DA, where it showed very acceptable analytical results. Additionally, the current PSi/GCE biosensor showed exceptional reproducibility, repeatability, and long-term stability.

首次研究了介孔硅 (PSi) 修饰的玻碳电极(GCE) 以有效检测多巴胺 (DA)。一种简便的染色蚀刻方法用于合成单晶 PSi 纳米颗粒，这一点得到了 SAED 的证实。通过 FESEM 和 TEM 对 PSi NPs 的形态研究表明，孔径小于 25 nm 的孔是随机分布的。X 射线衍射 (XRD)、拉曼光谱、傅立叶变换红外光谱 (FTIR) 和 X 射线光电子能谱 (XPS) 也用于表征 PSi NP。制造的PSi NPs修饰的GCE生物传感器可以测量磷酸盐缓冲溶液（PBS）中的多种多巴胺（0.5-333.3 μM），灵敏度值为0.2715 μAμM ^-1 cm⁻²的极低检测限 (LOD) 为 3.2 nM。这种非酶促多巴胺生物传感器还测试了常见干扰物质的可能影响，显示出非常好的选择性。目前的修饰电极进一步用于分析人血清和多巴胺盐酸盐注射液样品以检测DA，显示出非常可接受的分析结果。此外，当前的 PSi/GCE 生物传感器显示出卓越的重现性、可重复性和长期稳定性。