Magnaporthe oryzae is the causal agent of rice blast disease resulting in yield losses in rice-growing regions worldwide, particularly in Vietnam. Up-regulation of osa-miR7695 was observed in blast resistant rice cultivars in comparison with blast non-resistant rice cultivars. The OsNramp6 gene is a target for osa-miR7695, and considered a factor involved in rice immunity but scant evidence has demonstrated systematic expression profiles of OsNramp6 transcript variants involving M. oryzae susceptibility and resistance of rice plants. In this study, a real-time qPCR (qRT-PCR) approach was used to evaluate the expression levels of eight OsNramp6 transcript variants in various Vietnamese rice cultivars. Results indicated that OsNramp6.1 and OsNramp6.4 transcript variants showed decreased expression levels at 24 hpi in M. oryzae resistant rice cultivars and vice versa in the rice group of M. oryzae resistance, suggesting that both could be used as potential markers for discrimination between blast resistant and non-resistant Vietnamese rice cultivars. Identification of potential microRNAs in rice that contribute to the regulation of OsNramp6 transcripts-mediated facilitation of infection by M. oryzae has still to be confirmed for elucidating their roles in rice immunity against M. oryzae.

Magnaporthe oryzae是稻瘟病的病原体，导致世界各地水稻种植区的产量损失，特别是在越南。的上调OSA-miR7695在抗稻瘟病水稻品种观察到与高炉无抗性水稻品种比较。所述OsNramp6基因为目标OSA-miR7695，和认为参与免疫大米但充分证据的因子已经证明系统的表达谱OsNramp6转录物变体涉及稻瘟病菌的易感性和水稻植物的抗性。在这项研究中，实时 qPCR (qRT-PCR) 方法用于评估八种OsNramp6的表达水平各种越南水稻品种的转录变体。结果表明，OsNramp6.1和OsNramp6.4转录变体在 24 hpi 时在稻瘟病抗性水稻品种中表达水平降低，反之在稻瘟病抗性水稻组中表达水平降低，表明两者均可作为潜在的鉴别标志物抗稻瘟病和非抗稻瘟病的越南水稻品种之间的差异。水稻中有助于调节OsNramp6转录物介导的米曲霉感染的潜在 microRNA 的鉴定仍有待证实，以阐明它们在水稻针对米曲霉的免疫中的作用。