The enzymatic synthesis of hybrid Lewis antigens including KH-1 (Lewis y-Lewis x-Lactose, Le^y-Le^x-Lac), Lewis a-Lewis x-Lactose (Le^a-Le^x-Lac), and Lewis b-Lewis x-Lactose (Le^b-Le^x-Lac) has been achieved using a facile enzymatic modular assembly strategy. Starting from a readily available tetrasaccharide, 3 complex hybrid Lewis antigens were achieved in over 40% total yields in less than 5 linear steps of sequential enzymatic glycosylation using 6 enzyme modules. The regio-selective fucosylation was achieved by simply controlling the donor–acceptor ratio. This strategy provides an easy access to these biologically important complex hybrid Lewis antigens at preparative scales.

中文翻译：

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杂合路易斯抗原的酶模块化组装

混合 Lewis 抗原的酶促合成，包括 KH-1 (Lewis y-Lewis x-Lactose、Le ^y -Le ^x -Lac)、Lewis a-Lewis x-Lactose (Le ^a -Le ^x -Lac) 和 Lewis b- Lewis x-Lactose (Le ^b -Le ^x -Lac) 已使用简便的酶模块化组装策略实现。从容易获得的四糖开始，使用 6 个酶模块在少于 5 个线性连续酶促糖基化步骤中以超过 40% 的总产率获得了 3 个复杂的杂合路易斯抗原。通过简单地控制供体-受体比率来实现区域选择性岩藻糖基化。该策略提供了在制备规模上轻松获得这些生物学上重要的复杂杂合路易斯抗原的途径。