Approaches to Heterogeneity in Native Mass Spectrometry,Chemical Reviews

当前位置： X-MOL 学术 › Chem. Rev. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Approaches to Heterogeneity in Native Mass Spectrometry
Chemical Reviews ( IF 51.4 ) Pub Date : 2021-09-01 , DOI: 10.1021/acs.chemrev.1c00696
Amber D Rolland ₁ , James S Prell _{1,

2}

Affiliation

Native mass spectrometry (MS) is aimed at preserving and determining the native structure, composition, and stoichiometry of biomolecules and their complexes from solution after they are transferred into the gas phase. Major improvements in native MS instrumentation and experimental methods over the past few decades have led to a concomitant increase in the complexity and heterogeneity of samples that can be analyzed, including protein–ligand complexes, protein complexes with multiple coexisting stoichiometries, and membrane protein–lipid assemblies. Heterogeneous features of these biomolecular samples can be important for understanding structure and function. However, sample heterogeneity can make assignment of ion mass, charge, composition, and structure very challenging due to the overlap of tens or even hundreds of peaks in the mass spectrum. In this review, we cover data analysis, experimental, and instrumental advances and strategies aimed at solving this problem, with an in-depth discussion of theoretical and practical aspects of the use of available deconvolution algorithms and tools. We also reflect upon current challenges and provide a view of the future of this exciting field.

中文翻译：

天然质谱中的异质性方法

天然质谱 (MS) 旨在保存和确定溶液中的生物分子及其复合物转移到气相后的天然结构、组成和化学计量。过去几十年来，原生 MS 仪器和实验方法的重大改进导致可分析样品的复杂性和异质性随之增加，包括蛋白质-配体复合物、具有多种共存化学计量的蛋白质复合物以及膜蛋白-脂质组件。这些生物分子样品的异质特征对于理解结构和功能非常重要。然而，由于质谱中数十甚至数百个峰的重叠，样品的异质性可能使离子质量、电荷、组成和结构的分配变得非常具有挑战性。在这篇综述中，我们介绍了旨在解决该问题的数据分析、实验和仪器进展以及策略，并深入讨论了可用反卷积算法和工具的使用的理论和实践方面。我们还反思了当前的挑战，并展望了这个令人兴奋的领域的未来。

更新日期：2021-09-01

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11