In this study, a novel, low-cost, and flexible paper-based electrochemical immunosensor was developed for the bioanalysis of Cyfra 21.1 biomarker in human saliva samples by using stabilization of synthesis Ag nano-ink on the surface of paper using pen-on-paper technology. The employed electrochemical techniques for the evaluation of immunoplatform performance were differential pulse voltammetry and chronoamperometry. Also, the prepared immunosensor showed great ability in the determination of Cyfra21.1 in human saliva specimens. Under the optimized conditions, the obtained linear range was from 0.0025 to 10 ng/mL, and the obtained LLOQ was 0.0025 ng/mL. The developed immunosensor is easy to prepare, sensitive, cost-effective, portable, and simple. So proposed immunoplatform can be an accomplished biodevice in clinical laboratories. The proposed paper-based immunosensor could be a hopefully new and cheap tool for the diagnosis of other biomarkers. Also, the prepared immunosensor showed great ability in the determination of Cyfra21.1 biomarker in human saliva specimens.

中文翻译：

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电化学免疫平台通过测定人类唾液样本中的 CYFRA 21.1 生物标志物来辅助诊断口腔癌：制备一种新型便携式生物传感器，用于口腔癌的无创诊断

在这项研究中，开发了一种新型、低成本、灵活的纸基电化学免疫传感器，通过使用笔在纸表面稳定合成 Ag 纳米墨水，对人体唾液样本中的 Cyfra 21.1 生物标志物进行生物分析。纸技术。用于评估免疫平台性能的电化学技术是差分脉冲伏安法和计时电流法。此外，所制备的免疫传感器在检测人唾液标本中的 Cyfra21.1 方面表现出很强的能力。在优化条件下，得到的线性范围为0.0025~10 ng/mL，得到的LLOQ为0.0025 ng/mL。开发的免疫传感器易于制备、灵敏、成本效益高、便携且简单。因此，提出的免疫平台可以成为临床实验室中成熟的生物设备。所提出的纸基免疫传感器有望成为诊断其他生物标志物的新型廉价工具。此外，制备的免疫传感器在检测人唾液标本中的 Cyfra21.1 生物标志物方面表现出很强的能力。