Background

Meloidogyne arenaria is an economically important root-knot nematode (RKN) species whose hosts include maize (Zea mays). The plant response to RKN infection activates many cellular mechanisms, among others, changes in the expression level of genes encoding transcription and elongation factors as well as proteins related to cell wall organization.

Methods and results

This study is aimed at characterization of expression of selected transcription and elongation factors encoding the genes WRKY53, EF1a, and EF1b as well as the ones encoding two proteins associated with cell wall functioning (glycine-rich RNA-binding protein, GRP and polygalacturonase, PG) during the maize response to M. arenaria infection. The changes in the relative level of expression of genes encoding these proteins were assessed using the reverse transcription-quantitative real-time PCR. The material studied were leaves and root samples collected from four maize varieties showing different susceptibilities toward M. arenaria infection, harvested at three different time points. Significant changes in the expression level of GRP between susceptible and tolerant varieties were observed.

Conclusions

Results obtained in the study suggest pronounced involvement of glycine-rich RNA-binding protein and EF1b in the maize response and resistance to RKN.

中文翻译：

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玉米（Zea mays）根结线虫感染过程中编码转录因子和细胞壁相关蛋白基因表达水平的变化

背景

根结线虫是一种经济上重要的根结线虫 (RKN) 物种，其宿主包括玉米 ( Zea mays )。植物对 RKN 感染的反应激活了许多细胞机制，其中包括编码转录和延伸因子的基因以及与细胞壁组织相关的蛋白质的表达水平的变化。

方法和结果

本研究旨在表征编码基因WRKY53、EF1a和EF1b的选定转录和延伸因子的表达，以及编码与细胞壁功能相关的两种蛋白质（富含甘氨酸的 RNA 结合蛋白、GRP和多聚半乳糖醛酸酶、PG ）的表达。 ) 在玉米对M. arenararia感染的反应期间。使用逆转录定量实时 PCR 评估编码这些蛋白质的基因的相对表达水平的变化。研究的材料是从四种玉米品种中采集的叶子和根部样本，这些玉米品种对M. arearia具有不同的敏感性感染，在三个不同的时间点收获。观察到敏感品种和耐受品种之间GRP表达水平的显着变化。

结论

研究中获得的结果表明，富含甘氨酸的 RNA 结合蛋白和EF1b明显参与玉米对 RKN 的反应和抗性。