Artificial stabilization of the fusion pore by intra-organelle styrene–maleic acid copolymers,Soft Matter

当前位置： X-MOL 学术 › Soft Matter › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Artificial stabilization of the fusion pore by intra-organelle styrene–maleic acid copolymers
Soft Matter ( IF 2.9 ) Pub Date : 2021-08-16 , DOI: 10.1039/d1sm00978h
Marcelo Caparotta ₁ , Marcelo Puiatti ₂ , Diego Masone _{3,

4}

Affiliation

Styrene–maleic acid copolymers have become an advantageous detergent-free alternative for membrane protein isolation. Since their discovery, experimental membrane protein extraction and purification by keeping intact their lipid environment has become significantly easier. With the aim of identifying new applications of these interesting copolymers, their molecular binding and functioning mechanisms have recently become intense objects of study. In this work, we describe the use of styrene–maleic acid copolymers as an artificial tool to stabilize the fusion pore. We show that when these copolymers circumscribe the water channel that defines the fusion pore, they keep it from shrinking and closing. We describe how only intra-organelle copolymers have stabilizing capabilities while extra-organelle ones have negligible or even contrary effects on the fusion pore life-time.

中文翻译：

通过细胞器内苯乙烯-马来酸共聚物人工稳定融合孔

苯乙烯-马来酸共聚物已成为膜蛋白分离的一种有利的无洗涤剂替代品。自从他们的发现以来，通过保持脂质环境完整来进行实验性膜蛋白提取和纯化变得更加容易。为了确定这些有趣共聚物的新应用，它们的分子结合和功能机制最近已成为研究的热点。在这项工作中，我们描述了使用苯乙烯-马来酸共聚物作为稳定融合孔的人工工具。我们表明，当这些共聚物包围定义融合孔的水通道时，它们会阻止它收缩和关闭。

更新日期：2021-09-01

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11