Lepturus repens (G. Forst.) R. Br. is a perennial grass that grows on coral reef outcrops and sandy beaches and has drought tolerance and salt resistance. In this study, the internodes of L. repens were used as explants and cultured on Murashige and Skoog (MS) medium supplemented with 1.13 mg L⁻¹ 6-benzyladenine (BA) to successfully induce axillary shoots. Additional experimental results showed that cytokinins were a key factor for the successful induction and proliferation of shoots. BA induced 7.2 axillary shoots per explant, significantly more than kinetin (KIN), which induced 3.5 axillary shoots per explant. Shoots were proliferated more successfully on medium supplemented with 1.13 mg L⁻¹ BA and a low concentration of 0.09 mg L⁻¹ 1-naphthaleneacetic acid (NAA) than BA alone. Shoot bases were used as explants for embryogenic callus induction and subsequent plant regeneration, which was possible on MS medium supplemented with 0.11 mg L⁻¹ 2,4-D, 0.09 mg L⁻¹ NAA, and 0.22 mg L⁻¹ TDZ. Separately, callus differentiated into adventitious shoot buds on MS medium containing 1.13 mg L⁻¹ BA or 1.10 mg L⁻¹ KIN singly or combined with 0.09 mg L⁻¹ NAA. On MS medium without plant growth regulators (PGR-free) or only containing 0.09 mg L⁻¹ NAA, callus induced both adventitious shoots and somatic embryos that developed a radical on PGR-free medium. All shoots formed roots on ½MS medium containing a low concentration (0.19 to 0.37 mg L⁻¹) of NAA or IBA within 20 d. The survival percentage of plantlets with 3 to 4 shoot clusters exceeded 99% on a substrate consisting of yellow mud, organic fertilizer, and perlite (10:3:1, v:v:v). After transplanting, plantlets with one or two shoot clusters showed 68 to 86% survival within 1 mo.

Lepturus repens (G. Forst.) R. Br. 是一种多年生草本植物，生长在珊瑚礁露头和沙滩上，具有耐旱和耐盐性。在这项研究中，节间L.三叶作为外植体并在Murashige和补充有1.13毫克的L斯库格（MS）培养基中培养^-1 6-苄基腺嘌呤（BA），以成功诱导腋生芽。额外的实验结果表明，细胞分裂素是成功诱导和增殖芽的关键因素。BA 诱导每个外植体 7.2 个腋芽，显着超过激动素 (KIN)，每个外植体诱导 3.5 个腋芽。在补充有 1.13 mg L ^-1 BA 和 0.09 mg L 低浓度的培养基上更成功地增殖芽^-1 1-萘乙酸 (NAA) 而不是单独的 BA。茎基用作胚发生愈伤组织诱导和随后的植物再生的外植体，这在补充有0.11mg L ^-1 2,4-D、0.09mg L ^-1 NAA和0.22mg L ^-1 TDZ的MS培养基上是可能的。单独地，愈伤组织在含有1.13 mg L ^-1 BA 或1.10 mg L ^-1 KIN 单独或与0.09 mg L ^-1 NAA组合的MS 培养基上分化为不定芽芽。在不含植物生长调节剂（无 PGR）或仅含 0.09 mg L ^{-1 的}MS 培养基上NAA、愈伤组织诱导不定枝和体细胞胚，它们在无 PGR 培养基上产生自由基。所有芽在含有低浓度（0.19 至 0.37 mg L ^-1）的 NAA 或 IBA 的½MS 培养基上在 20 天内形成根。在由黄泥、有机肥和珍珠岩(10:3:1, v : v : v )组成的基质上，具有3至4个枝条簇的小苗成活率超过99% 。移植后，具有一到两个枝条簇的小植株在 1 个月内显示出 68% 到 86% 的存活率。