Development of Ultranarrow-Bore Open Tubular High Efficiency Liquid Chromatography,Chinese Journal of Chemistry

当前位置： X-MOL 学术 › Chin. J. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Development of Ultranarrow-Bore Open Tubular High Efficiency Liquid Chromatography
Chinese Journal of Chemistry ( IF 5.5 ) Pub Date : 2021-09-01 , DOI: 10.1002/cjoc.202100445
Yingyan Zhou ₁ , Guangsheng Guo ₁ , Xiayan Wang ₁

Affiliation

Chromatography theory shows that when the inner diameter of the chromatography column is very narrow (e.g., 1—2 μm), the maximum efficiency will be produced in liquid chromatography. In addition, the increasing demand for ultrasmall volume sample analysis has produced the trend of narrowing the diameter of the liquid chromatography column. Experiments have verified that ultranarrow-bore open tubular liquid chromatography (i.d. ≤ 2 μm) has the advantages of small sample requirement and high separation efficiency. However, there also exist huge challenges along with such obvious advantages. This review summarizes the efforts made by our research group and other research groups to develop this field. We hope that in the near future, ultranarrow bore open-tube liquid chromatography can be successfully and maturely applied to the application of analytes with ultrasmall size and volume such as single-cell and even subcellular organelles omics research.

中文翻译：

超窄孔开管高效液相色谱的研制

色谱理论表明，当色谱柱的内径很窄时（例如., 1-2 μm)，液相色谱法的效率最高。此外，对超小体积样品分析的需求不断增加，也产生了液相色谱柱管径变窄的趋势。实验证明超窄孔开管液相色谱（内径≤2 μm）具有样品量少、分离效率高等优点。然而，在如此明显的优势的同时，也存在着巨大的挑战。本综述总结了我们的研究小组和其他研究小组为发展这一领域所做的努力。我们希望在不久的将来，超窄孔开管液相色谱能够成功并成熟地应用于单细胞甚至亚细胞细胞器组学研究等超小体积和超小体积分析物的应用。

更新日期：2021-09-01

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11