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Study of anesthetics for euthanasia in rats and mice: A systematic review and meta-analysis on the impact upon biological outcomes (SAFE-RM)
Life Sciences ( IF 5.2 ) Pub Date : 2021-09-01 , DOI: 10.1016/j.lfs.2021.119916
Santiago Alonso Tobar Leitão 1 , Douglas Dos Santos Soares 1 , Nelson Carvas Junior 2 , Rafael Zimmer 3 , Nataniel Floriano Ludwig 4 , Michael Andrades 1
Affiliation  

To summarize the knowledge on the effect of anesthetics employed right before euthanasia on biological outcomes. A systematic review of the literature to find studies with isoflurane, ketamine, halothane, pentobarbital, or thiopental just before euthanasia of laboratory rats or mice. Controlled studies with quantitative data available. The search, data extraction, and risk of bias (RoB) were performed independently by two reviewers using a structured form. For each outcome, an effect size (ES) was calculated relative to the control group. Meta-analysis was performed using robust variance meta-regression for hierarchical data structures, with adjustment for small samples. We included 20 studies with 407 biological outcomes (110 unique). RoB analysis indicated that 87.5% of the domains evaluated showed unclear risk, 2% high risk, and 10.5% low risk. The effect size for all anesthetics considered together was 0.99 (CI = 0.75–1.23; < 0.0001). Sub-analyses indicate high effect sizes for pentobarbital (1.14; CI = 0.75–1.52; p < 0.0001), and isoflurane (1.01; CI = 0.58–1.44; = 0.0005) but not for ketamine (1.49; CI = -7.95–10.9; = 0.295). We showed that anesthetics interfere differently with the majority of the outcomes assessed. However, our data did not support the use of one anesthetic over others or even the killing without anesthetics. We conclude that outcomes cannot be compared among studies without considering the killing method. This protocol was registered at Prospero (CRD42019119520). Funding: There was no direct funding for this research.

中文翻译:


大鼠和小鼠安乐死麻醉剂的研究:对生物学结果影响的系统回顾和荟萃分析 (SAFE-RM)



总结关于安乐死前使用的麻醉剂对生物学结果影响的知识。对文献进行系统回顾,查找在实验室大鼠或小鼠安乐死之前使用异氟烷、氯胺酮、氟烷、戊巴比妥或硫喷妥钠的研究。具有可用定量数据的对照研究。搜索、数据提取和偏倚风险 (RoB) 由两名评审员使用结构化表格独立执行。对于每个结果,相对于对照组计算效应大小(ES)。使用针对分层数据结构的稳健方差元回归进行荟萃分析,并针对小样本进行调整。我们纳入了 20 项研究,涉及 407 项生物学结果(110 项是独特的)。 RoB 分析表明,87.5% 的评估领域显示出不明确的风险,2% 为高风险,10.5% 为低风险。所有麻醉剂的效应大小一起考虑为 0.99 (CI = 0.75–1.23; < 0.0001)。子分析表明戊巴比妥 (1.14;CI = 0.75–1.52;p < 0.0001) 和异氟烷 (1.01;CI = 0.58–1.44;= 0.0005) 具有较高的效应值,但氯胺酮 (1.49;CI = -7.95–10.9) 的效应值不高; = 0.295)。我们发现麻醉剂对大多数评估结果的干扰不同。然而,我们的数据并不支持使用一种麻醉剂优于其他麻醉剂,甚至不支持在不使用麻醉剂的情况下杀人。我们的结论是,如果不考虑灭杀方法,则无法对研究之间的结果进行比较。该协议已在 Prospero 注册(CRD42019119520)。资助:这项研究没有直接资助。
更新日期:2021-09-01
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