In human cardiovascular research, sheep in particular are used as a large animal model in addition to pigs. In these animals, medical products, developed and tested for human medical purposes, are almost exclusively used in interventional studies. Therefore, the extent to which platelets from humanand ovine blood differ in terms of adherence, aggregation and activation after a 4- or 8-minutes exposure to glass was investigated. Testing was performed with platelet-rich plasma (PRP) and a modified Chandler loop system, with 4- and 8-minute blood-material exposure times corresponding to 20 and 40 test cycles, respectively, through the entire silicon tube loop of the test system. In sheep and human PRP, contact with the silicone tubing resulted in a decrease in platelet count after 4 minutes and 20 test cycles, respectively. Four more minutes (20 additional test cycles) caused a further decrease of the platelet count only in sheep PRP. When the silicon tube was partly filled with glass beads, these effects were more pronounced and stronger in sheep then in human PRP. The mean platelet volume, which was used as parameter for platelet aggregation, did not change over time in human PRP without glass exposure. With glass exposure in human and sheep PRP the mean platelet volume increased within 40 test cycles, but this increase was stronger in sheep than in human PRP. Regarding activation behavior, the activation markers CD62P and CD63 were detectable only in < 30% (sheep) and < 45% (human) of platelets, whereas after 8 min of glass exposure, the proportion of CD62P+ and CD63+ cells was more increased than before only in sheep. These results indicate that ovine platelets adhere more strongly to glass and show stronger aggregation behavior after glass contact than human platelets, but that ovine and human platelets differ only slightly in activability by glass.

中文翻译：

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玻璃珠在改良钱德勒环系统中诱导人和羊血小板粘附、聚集和活化的差异

在人类心血管研究中，除了猪之外，绵羊尤其被用作大型动物模型。在这些动物中，为人类医疗目的开发和测试的医疗产品几乎只用于介入研究。因此，研究了人血和羊血中的血小板在暴露于玻璃 4 或 8 分钟后在粘附、聚集和活化方面的差异程度。使用富含血小板的血浆 (PRP) 和改良的 Chandler 环路系统进行测试，通过测试系统的整个硅管环路，分别对应 20 和 40 个测试周期的 4 分钟和 8 分钟血液材料暴露时间. 在绵羊和人类 PRP 中，与硅胶管接触分别导致 4 分钟和 20 个测试周期后血小板计数减少。仅在绵羊 PRP 中，再多四分钟（20 个额外的测试周期）导致血小板计数进一步减少。当硅管中部分填充玻璃珠时，这些效果在绵羊中比在人 PRP 中更明显和更强。用作血小板聚集参数的平均血小板体积在没有玻璃暴露的人 PRP 中不会随时间变化。在人和绵羊 PRP 的玻璃暴露下，平均血小板体积在 40 个测试周期内增加，但羊的这种增加比人 PRP 更强。关于激活行为，激活标记 CD62P 和 CD63 仅在 < 30%（绵羊）和 < 45%（人）的血小板中检测到，而在玻璃暴露 8 分钟后，CD62P+ 和 CD63+ 细胞的比例比以前增加更多只在羊身上。